+Open data
-Basic information
Entry | Database: PDB / ID: 4bhv | ||||||
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Title | Measles virus phosphoprotein tetramerization domain | ||||||
Components | PHOSPHOPROTEIN | ||||||
Keywords | VIRAL PROTEIN / OLIGOMERIZATION DOMAIN | ||||||
Function / homology | Function and homology information viral genome replication / RNA-dependent RNA polymerase activity / DNA-templated transcription / RNA binding Similarity search - Function | ||||||
Biological species | MEASLES VIRUS | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Blocquel, D. / Habchi, J. / Durand, E. / Sevajol, M. / Ferron, F. / Papageorgiou, N. / Longhi, S. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2014 Title: Coiled-Coil Deformations in Crystal Structures: The Measles Virus Phosphoprotein Multimerization Domain as an Illustrative Example. Authors: Blocquel, D. / Habchi, J. / Durand, E. / Sevajol, M. / Ferron, F. / Erales, J. / Papageorgiou, N. / Longhi, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bhv.cif.gz | 61.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bhv.ent.gz | 47.1 KB | Display | PDB format |
PDBx/mmJSON format | 4bhv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4bhv_validation.pdf.gz | 467.4 KB | Display | wwPDB validaton report |
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Full document | 4bhv_full_validation.pdf.gz | 473.1 KB | Display | |
Data in XML | 4bhv_validation.xml.gz | 12.9 KB | Display | |
Data in CIF | 4bhv_validation.cif.gz | 17.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bh/4bhv ftp://data.pdbj.org/pub/pdb/validation_reports/bh/4bhv | HTTPS FTP |
-Related structure data
Related structure data | 4c5qC 1ezjS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7505.484 Da / Num. of mol.: 4 / Fragment: TETRAMERIZATION DOMAIN, RESIDUES 304-360 / Mutation: YES Source method: isolated from a genetically manipulated source Details: TITLE IS-MEASLES VIRUS PHOSPHOPROTEIN TETRAMERIZATION DOMAIN Source: (gene. exp.) MEASLES VIRUS / Strain: EDMONSTON B / Plasmid: PDEST14 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA PLYSS / References: UniProt: P35974 #2: Chemical | ChemComp-ACT / #3: Chemical | ChemComp-CA / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 32.55 % / Description: NONE |
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Crystal grow | pH: 5.5 / Details: PH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.976254 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976254 Å / Relative weight: 1 |
Reflection twin | Operator: H,-K,-H-L / Fraction: 0.12 |
Reflection | Resolution: 1.76→32.8 Å / Num. obs: 21142 / % possible obs: 97.1 % / Observed criterion σ(I): 2 / Redundancy: 3.3 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 7.32 |
Reflection shell | Resolution: 1.76→1.82 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 1.94 / % possible all: 87 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1EZJ Resolution: 2.1→32.848 Å / σ(F): 1.4
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→32.848 Å
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Refine LS restraints |
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LS refinement shell |
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