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- PDB-4a4s: UNAC Tetraloops: To What Extent Can They Mimic GNRA Tetraloops -

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Basic information

Entry
Database: PDB / ID: 4a4s
TitleUNAC Tetraloops: To What Extent Can They Mimic GNRA Tetraloops
Components5'-R(*GP*GP*AP*CP*CP*CP*GP*GP*CP*UP*CP*AP*CP*GP *CP*UP*GP*GP*GP*UP*CP*C)-3'
KeywordsRNA / EVOLUTION / UMAC TETRALOOP
Function / homologyRNA / RNA (> 10)
Function and homology information
Biological speciesTHERMUS THERMOPHILUS (bacteria)
MethodSOLUTION NMR
AuthorsZhao, Q. / Huang, H. / Nagaswamy, U. / Xia, Y. / Gao, X. / Fox, G.
CitationJournal: Biopolymers / Year: 2012
Title: Unac Tetraloops: To What Extent Can They Mimic Gnra Tetraloops
Authors: Zhao, Q. / Huang, H. / Nagaswamy, U. / Xia, Y. / Gao, X. / Fox, G.
History
DepositionOct 20, 2011Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 30, 2012Provider: repository / Type: Initial release
Revision 1.1Apr 19, 2017Group: Other
Revision 1.2May 15, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_nmr_spectrometer
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_spectrometer.model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 5'-R(*GP*GP*AP*CP*CP*CP*GP*GP*CP*UP*CP*AP*CP*GP *CP*UP*GP*GP*GP*UP*CP*C)-3'


Theoretical massNumber of molelcules
Total (without water)7,0401
Polymers7,0401
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)7 / -
Representative

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Components

#1: RNA chain 5'-R(*GP*GP*AP*CP*CP*CP*GP*GP*CP*UP*CP*AP*CP*GP *CP*UP*GP*GP*GP*UP*CP*C)-3' / UCAC


Mass: 7040.234 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) THERMUS THERMOPHILUS (bacteria)

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: NONE

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Sample preparation

DetailsContents: 99.96% D2O
Sample conditionspH: 7 / Temperature: 298.0 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE6001
Bruker AVANCEBrukerAVANCE8002

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Processing

NMR softwareName: CNS
Developer: BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARREN
Classification: refinement
NMR ensembleConformers submitted total number: 7

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