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- PDB-469d: CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-ME... -
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Open data
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Basic information
Entry | Database: PDB / ID: 469d | ||||||||||||||||||
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Title | CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA | ||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / 2'-O-(2-METHOXYETHYL) RIBOSE / RNA DODECAMER DUPLEX | Function / homology | RNA / RNA (> 10) | ![]() Method | ![]() ![]() ![]() Teplova, M. / Minasov, G. / Tereshko, V. / Inamati, G. / Cook, P.D. / Egli, M. | ![]() ![]() Title: Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA. Authors: Teplova, M. / Minasov, G. / Tereshko, V. / Inamati, G.B. / Cook, P.D. / Manoharan, M. / Egli, M. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28.1 KB | Display | ![]() |
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PDB format | ![]() | 22.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 399.3 KB | Display | ![]() |
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Full document | ![]() | 408.4 KB | Display | |
Data in XML | ![]() | 6.5 KB | Display | |
Data in CIF | ![]() | 7.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: RNA chain | Mass: 4524.284 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: O2* OF EACH RIBOSE MODIFIED BY METHOXYETHYL #2: Chemical | ChemComp-MG / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.83 Å3/Da / Density % sol: 32.64 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: DROPLETS CONTAINING 2MM OLIGONUCLEOTIDE, 50 MM MGCL2, 50 MM NA HEPES PH 6.2, 15% PEG 400, AGAINST 1 ML OF RESERVOIR SOLUTION CONTAINING 100 MM MGCL2, 100 MM NA HEPES PH 6.2, 30% PEG 400, ...Details: DROPLETS CONTAINING 2MM OLIGONUCLEOTIDE, 50 MM MGCL2, 50 MM NA HEPES PH 6.2, 15% PEG 400, AGAINST 1 ML OF RESERVOIR SOLUTION CONTAINING 100 MM MGCL2, 100 MM NA HEPES PH 6.2, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 295K | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 7, 1998 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→20 Å / Num. all: 7032 / Num. obs: 7032 / % possible obs: 99.4 % / Observed criterion σ(I): 0 / Redundancy: 5.6 % / Rmerge(I) obs: 0.055 |
Reflection | *PLUS Num. measured all: 39271 |
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Processing
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Refinement | Resolution: 1.7→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.7→8 Å
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Refine LS restraints |
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