- PDB-3zfj: N-terminal domain of pneumococcal PhtD protein with bound Zn(II) -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3zfj
Title
N-terminal domain of pneumococcal PhtD protein with bound Zn(II)
Components
PNEUMOCOCCAL HISTIDINE TRIAD PROTEIN D
Keywords
ZINC-BINDING PROTEIN / ZINC TRANSFER
Function / homology
Streptococcal histidine triad repeat / Histidine triad protein / PhtA domain superfamily / Streptococcal histidine triad protein / Pneumococcal histidine triad protein D / Pneumococcal histidine triad protein D
Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
Sequence details
RESIDUES 2-138 OF THE T-PHTD SEQUENCE CORRESPOND TO RESIDUES 30-166 OF THE Q04KS8 ENTRY
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
15N-HSQC
1
2
1
13C-HSQC
1
3
1
3D-15N- NOESY
1
4
1
3D-13C-NOESY
2
5
2
2D- NOESY
NMR details
Text: BACKBONE ASSIGNMENT FROM STANDARD TRIPLE RESONANCE EXPERIMENTS. CCONH-TOCSY AND 3D-NOESY EXPERIMENTS FOR SIDECHAIN ASSIGNMENT. 2 3D-NOESY AND A 2D-NOESY IN D2O FOR THE EXTRACTION OF DISTANCE CONSTRAINTS.
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Sample preparation
Details
Solution-ID
Contents
1
90% WATER, 10% D2O
2
100% D2O
Sample conditions
Ionic strength: 50 mM / pH: 6.3 / Pressure: 1 atm / Temperature: 308.0 K
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