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- PDB-3wdi: Crystal structure of Pullulanase complexed with maltotriose from ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3wdi | |||||||||
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Title | Crystal structure of Pullulanase complexed with maltotriose from Anoxybacillus sp. LM18-11 | |||||||||
![]() | Type I pullulanase | |||||||||
![]() | HYDROLASE / glycoside hydrolase / pullulanase | |||||||||
Function / homology | ![]() pullulanase / pullulanase activity / carbohydrate metabolic process / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Xu, J. / Ren, F. / Huang, C.H. / Zheng, Y. / Zhen, J. / Ko, T.P. / Chen, C.C. / Chan, H.C. / Guo, R.T. / Ma, Y. / Song, H. | |||||||||
![]() | ![]() Title: Cloning, Expression, Functional and Structural Studies of Pullulanase from Anoxybacillus sp. LM18-11 Authors: Xu, J. / Ren, F. / Huang, C.H. / Zheng, Y. / Zhen, J. / Chen, C.C. / Chan, H.C. / Guo, R.T. / Ma, Y. / Song, H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 171.2 KB | Display | ![]() |
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PDB format | ![]() | 133 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 33.6 KB | Display | |
Data in CIF | ![]() | 50.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3wdhSC ![]() 3wdjC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 82172.422 Da / Num. of mol.: 1 / Fragment: UNP residues 3-707 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Polysaccharide | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltotriose #3: Chemical | ChemComp-CA / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.52 % |
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Crystal grow | Temperature: 295 K / pH: 7.5 Details: 0.1M HEPES, pH 7.5, 7%(w/v) Polyethylene Glycol 8000, 10%(v/v) Ethylene Glycol , VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 20, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→25 Å / Num. obs: 43239 / % possible obs: 99.7 % / Redundancy: 7.2 % / Rmerge(I) obs: 0.082 / Net I/σ(I): 26.6 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.475 / Mean I/σ(I) obs: 4.5 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3WDH Resolution: 2.2→25 Å / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→25 Å
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LS refinement shell | Resolution: 2.2→2.28 Å /
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