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- PDB-3w5u: Cross-linked complex between Ferredoxin and Ferredoxin-NADP+ reductase -
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Open data
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Basic information
Entry | Database: PDB / ID: 3w5u | ||||||
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Title | Cross-linked complex between Ferredoxin and Ferredoxin-NADP+ reductase | ||||||
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![]() | ELECTRON TRANSPORT / electron transfer complex | ||||||
Function / homology | ![]() chloroplast thylakoid membrane protein complex / oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / response to light stimulus / photosynthesis / chloroplast / electron transport chain ...chloroplast thylakoid membrane protein complex / oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / response to light stimulus / photosynthesis / chloroplast / electron transport chain / 2 iron, 2 sulfur cluster binding / electron transfer activity / nucleotide binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kimata-Ariga, Y. / Kubota-Kawai, H. / Muraki, N. / Hase, T. / Kurisu, G. | ||||||
![]() | ![]() Title: Concentration-dependent oligomerization of cross-linked complexes between ferredoxin and ferredoxin-NADP(+) reductase Authors: Kimata-Ariga, Y. / Kubota-Kawai, H. / Lee, Y.-H. / Muraki, N. / Ikegami, T. / Kurisu, G. / Hase, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 314.4 KB | Display | ![]() |
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PDB format | ![]() | 255.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3w5vC ![]() 1gawS ![]() 3b2fS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 35334.477 Da / Num. of mol.: 4 / Fragment: UNP residues 42-355 / Mutation: E19C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 10534.435 Da / Num. of mol.: 4 / Fragment: UNP residues 53-150 / Mutation: S59C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | ChemComp-FAD / #4: Chemical | ChemComp-FES / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.75 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 20% PEG 6000, 50mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Dec 18, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.68→150 Å / Num. obs: 48206 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1GAW, 3B2F Resolution: 2.7→45.46 Å / Cor.coef. Fo:Fc: 0.896 / Cor.coef. Fo:Fc free: 0.839 / SU B: 13.835 / SU ML: 0.289 / Cross valid method: THROUGHOUT / ESU R Free: 0.406 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.921 Å2
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Refinement step | Cycle: LAST / Resolution: 2.7→45.46 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.699→2.769 Å / Total num. of bins used: 20
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