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- PDB-3tci: Crystal structure of the decameric sequence d(CGGGCGCCCG) as Z ty... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3tci | ||||||||||||||||||
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Title | Crystal structure of the decameric sequence d(CGGGCGCCCG) as Z type duplex | ||||||||||||||||||
![]() | (DNA (5'-D(P*![]() DNA / Z-DNA duplex | Function / homology | DNA | ![]() Method | ![]() ![]() ![]() Venkadesh, S. / Mandal, P.K. / Gautham, N. | ![]() ![]() Title: Crystal structure of the decameric sequence d(CGGGCGCCCG) as Z type duplex Authors: Venkadesh, S. / Mandal, P.K. / Gautham, N. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 15.3 KB | Display | ![]() |
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PDB format | ![]() | 10.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377.5 KB | Display | ![]() |
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Full document | ![]() | 377.8 KB | Display | |
Data in XML | ![]() | 2.8 KB | Display | |
Data in CIF | ![]() | 3.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 1191.818 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Chemically synthesized #2: DNA chain | Mass: 1191.818 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Chemically synthesized #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1mM DNA, 2M MgCl2, 1mM spermine, 50% MPD , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 2, 2009 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.41→30 Å / Num. all: 586 / Num. obs: 579 / % possible obs: 98.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.41 % / Biso Wilson estimate: 54.506 Å2 / Rmerge(I) obs: 0.061 / Rsym value: 0.053 / Net I/σ(I): 4.4 |
Reflection shell | Resolution: 2.41→2.5 Å / Redundancy: 3.51 % / Rmerge(I) obs: 0.271 / Mean I/σ(I) obs: 1.7 / Num. unique all: 63 / Rsym value: 0.224 / % possible all: 96.9 |
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Processing
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Refinement | Method to determine structure: ![]() Details: The sequence is d(CGGGCGCCCG). The molecule is placed in three-fold screw axis and form pseudo-continuous helix (Z-type) along c-axis. For this reason, the crystallographic asymmetric unit ...Details: The sequence is d(CGGGCGCCCG). The molecule is placed in three-fold screw axis and form pseudo-continuous helix (Z-type) along c-axis. For this reason, the crystallographic asymmetric unit consist of two tetramers such as d(CGCG) in 80% and d(GCGC) in 20%.
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 172.268 Å2 / ksol: 0.6 e/Å3 | |||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.421→15.387 Å
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Refine LS restraints |
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LS refinement shell | Highest resolution: 2.4213 Å
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