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- PDB-3ssa: Crystal structure of subunit B mutant N157T of the A1AO ATP synthase -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ssa | ||||||
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Title | Crystal structure of subunit B mutant N157T of the A1AO ATP synthase | ||||||
![]() | V-type ATP synthase beta chain | ||||||
![]() | HYDROLASE / ATP binding | ||||||
Function / homology | ![]() proton-transporting two-sector ATPase complex, catalytic domain / vacuolar acidification / proton motive force-driven plasma membrane ATP synthesis / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Sundararaman, L. / Manimekalai, M.S.S. / Jeyakanthan, J. / Gruber, G. | ||||||
![]() | ![]() Title: Structure of subunit A mutants H156A, N157A and N157T of the A1AO ATP synthase Authors: Sundararaman, L. / Manimekalai, M.S.S. / Gruber, G. #1: ![]() Title: Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase. Authors: Kumar, A. / Manimekalai, M.S. / Balakrishna, A.M. / Hunke, C. / Weigelt, S. / Sewald, N. / Gruber, G. #2: ![]() Title: Structure of the nucleotide-binding subunit B of the energy producer A1A0 ATP synthase in complex with adenosine diphosphate. Authors: Kumar, A. / Manimekalai, M.S. / Gruber, G. #3: ![]() Title: A second transient position of ATP on its trail to the nucleotide-binding site of subunit B of the motor protein A(1)A(0) ATP synthase. Authors: Manimekalai, M.S. / Kumar, A. / Balakrishna, A.M. / Gruber, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 216.4 KB | Display | ![]() |
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PDB format | ![]() | 168.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3tgwC ![]() 3tivC ![]() 2c61S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 50327.242 Da / Num. of mol.: 2 / Mutation: N157T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q60187, H+-transporting two-sector ATPase |
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-Non-polymers , 7 types, 1129 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/AES.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/AES.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-GOL / #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-AES / | #6: Chemical | ChemComp-PEG / #7: Chemical | ChemComp-PGE / | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.93 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 30% glycerol, PEG 400, 0.1M Sodium Chloride and 0.1M Sodium citrate (pH 5.0), vapor diffusion, hanging drop, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 7, 2010 / Details: mirrors | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Redundancy: 5.4 % / Av σ(I) over netI: 20.8 / Number: 552277 / Rmerge(I) obs: 0.044 / Χ2: 0.44 / D res high: 1.7 Å / D res low: 30 Å / Num. obs: 101545 / % possible obs: 99.5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
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Reflection | Resolution: 1.7→30 Å / Num. all: 102059 / Num. obs: 101545 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / Redundancy: 5.4 % / Biso Wilson estimate: 18.934 Å2 / Rmerge(I) obs: 0.044 / Χ2: 0.44 / Net I/σ(I): 7.8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2C61 Resolution: 1.7→13.45 Å / Cor.coef. Fo:Fc: 0.976 / Cor.coef. Fo:Fc free: 0.959 / WRfactor Rfree: 0.1916 / WRfactor Rwork: 0.1429 / Occupancy max: 1 / Occupancy min: 0.5 / FOM work R set: 0.8739 / SU B: 1.877 / SU ML: 0.062 / SU R Cruickshank DPI: 0.0898 / SU Rfree: 0.0969 / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.097 / Stereochemistry target values: Engh & Huber Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 78.41 Å2 / Biso mean: 25.3045 Å2 / Biso min: 7.48 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→13.45 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.697→1.74 Å / Total num. of bins used: 20
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