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Open data
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Basic information
Entry | Database: PDB / ID: 3s8m | ||||||
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Title | The Crystal Structure of FabV | ||||||
![]() | Enoyl-ACP Reductase | ||||||
![]() | OXIDOREDUCTASE / ROSSMANN FOLD / NADH Binding / FATTY ACID SYNTHESIS / enoyl-ACP | ||||||
Function / homology | ![]() trans-2-enoyl-CoA reductase (NAD+) / enoyl-[acyl-carrier-protein] reductase activity (NAD(P)H) / trans-2-enoyl-CoA reductase (NADH) activity / enoyl-[acyl-carrier-protein] reductase (NADH) / enoyl-[acyl-carrier-protein] reductase (NADH) activity / fatty acid biosynthetic process / NAD binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, H. / Zhang, X.L. / Bi, L.J. / He, J. / Jiang, T. | ||||||
![]() | ![]() Title: Determination of the Crystal Structure and Active Residues of FabV, the Enoyl-ACP Reductase from Xanthomonas oryzae. Authors: Li, H. / Zhang, X. / Bi, L. / He, J. / Jiang, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.2 KB | Display | ![]() |
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PDB format | ![]() | 70.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46247.066 Da / Num. of mol.: 1 / Mutation: E192D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: KS-1-21 / Gene: Xanthomonas oryzae, XOO0026 / Plasmid: pET28B / Production host: ![]() ![]() References: UniProt: Q2P9J6, Oxidoreductases; Acting on the CH-CH group of donors; With NAD+ or NADP+ as acceptor |
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#2: Water | ChemComp-HOH / |
Sequence details | AUTHORS STATE THAT THE FIRST TWO METHIONINES WERE NOT SEEN IN THE STRUCTURE. THEREFORE, COULD NOT ...AUTHORS STATE THAT THE FIRST TWO METHIONINE |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.73 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 100 mM Bis-Tris, 200 mM NaCl, 1 mM DTT and 15% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Jan 20, 2010 | |||||||||
Radiation | Monochromator: GRAPHITE / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.6→19.89 Å / Num. all: 53994 / Num. obs: 53994 / % possible obs: 99.5 % / Observed criterion σ(F): 2.13 / Redundancy: 14.3 % / Rmerge(I) obs: 0.06 / Rsym value: 0.205 / Net I/σ(I): 64.1 | |||||||||
Reflection shell | Resolution: 1.6→1.66 Å / Rmerge(I) obs: 0.184 / Mean I/σ(I) obs: 14.3 / Rsym value: 0.068 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 40.022 Å2 / ksol: 0.364 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.6→19.89 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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