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- PDB-3rap: The small G protein Rap2 in a non catalytic complex with GTP -

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Basic information

Entry
Database: PDB / ID: 3rap
TitleThe small G protein Rap2 in a non catalytic complex with GTP
ComponentsPROTEIN (G protein RAP2A)
KeywordsSIGNALING PROTEIN / G PROTEIN / RAS / GTPASE / RAP2
Function / homology
Function and homology information


Rap protein signal transduction / microvillus assembly / positive regulation of protein autophosphorylation / regulation of JNK cascade / regulation of dendrite morphogenesis / regulation of synapse assembly / regulation of postsynaptic membrane neurotransmitter receptor levels / negative regulation of cell migration / synaptic membrane / small monomeric GTPase ...Rap protein signal transduction / microvillus assembly / positive regulation of protein autophosphorylation / regulation of JNK cascade / regulation of dendrite morphogenesis / regulation of synapse assembly / regulation of postsynaptic membrane neurotransmitter receptor levels / negative regulation of cell migration / synaptic membrane / small monomeric GTPase / protein localization to plasma membrane / establishment of protein localization / recycling endosome / Schaffer collateral - CA1 synapse / cellular response to xenobiotic stimulus / recycling endosome membrane / positive regulation of protein phosphorylation / GDP binding / intracellular protein localization / G protein activity / actin cytoskeleton organization / midbody / GTPase activity / GTP binding / magnesium ion binding / plasma membrane / cytosol
Similarity search - Function
Ras-related protein Rap2 / Small GTPase, Ras-type / Small GTPase Ras domain profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain ...Ras-related protein Rap2 / Small GTPase, Ras-type / Small GTPase Ras domain profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Ras-related protein Rap-2a
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å
AuthorsMenetrey, J. / Cherfils, J.
Citation
Journal: Proteins / Year: 1999
Title: Structure of the small G protein Rap2 in a non-catalytic complex with GTP.
Authors: Menetrey, J. / Cherfils, J.
#1: Journal: Embo J. / Year: 1997
Title: Crystal Structures of the Small G Protein Rap2A in Complex with its Substrate GTP, with GDP and with GTPgS
Authors: Cherfils, J. / Menetrey, J. / Lebras, G. / Lebras, G. / Janoueix-Lerosey, I. / De Gunzburg, J. / Garel, J.R. / Auzat, I.
History
DepositionMay 31, 1999Deposition site: BNL / Processing site: RCSB
Revision 1.0Dec 8, 1999Provider: repository / Type: Initial release
Revision 1.1Apr 26, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Apr 4, 2018Group: Data collection / Category: diffrn_source / Item: _diffrn_source.type
Revision 1.4Apr 11, 2018Group: Data collection / Category: diffrn_source / Item: _diffrn_source.pdbx_synchrotron_beamline
Revision 1.5Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
R: PROTEIN (G protein RAP2A)
S: PROTEIN (G protein RAP2A)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)38,9866
Polymers37,8912
Non-polymers1,0954
Water1,67593
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)41.010, 59.830, 68.440
Angle α, β, γ (deg.)90.00, 97.07, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein PROTEIN (G protein RAP2A)


Mass: 18945.562 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P10114
#2: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#3: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 93 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.2 Å3/Da / Density % sol: 44.05 %
Crystal growpH: 8 / Details: PEG 8000 20-25% TRIS-HCL 100MM PH 8 100MM MGCL2
Crystal grow
*PLUS
Method: vapor diffusion, hanging drop
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDDetailsChemical formula
110 mg/mlprotein1drop
220-25 %PEG80001reservoir
3100 mMTris-HCl1reservoir
4100 mM1reservoiror MgCl2Li2SO4

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: BM02 / Wavelength: 0.9798
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9798 Å / Relative weight: 1
ReflectionResolution: 2.2→16 Å / Num. obs: 16388 / % possible obs: 97.5 % / Redundancy: 4 % / Biso Wilson estimate: 24.5 Å2 / Rsym value: 0.072 / Net I/σ(I): 15
Reflection
*PLUS
Num. measured all: 144323 / Rmerge(I) obs: 0.072

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Processing

Software
NameClassification
AMoREphasing
X-PLORrefinement
DENZOdata reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: RAP2A-GTP HEXAGONAL FORM (PDB 2RAP)

2rap


Resolution: 2.2→16 Å / σ(F): 2
RfactorNum. reflection% reflectionSelection details
Rfree0.262 -9 %RANDOM
Rwork0.211 ---
obs0.211 16388 97.5 %-
Displacement parametersBiso mean: 25 Å2
Refinement stepCycle: LAST / Resolution: 2.2→16 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2658 0 66 93 2817
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONx_bond_d0.008
X-RAY DIFFRACTIONx_bond_d_na
X-RAY DIFFRACTIONx_bond_d_prot
X-RAY DIFFRACTIONx_angle_d
X-RAY DIFFRACTIONx_angle_d_na
X-RAY DIFFRACTIONx_angle_d_prot
X-RAY DIFFRACTIONx_angle_deg2.065
X-RAY DIFFRACTIONx_angle_deg_na
X-RAY DIFFRACTIONx_angle_deg_prot
X-RAY DIFFRACTIONx_dihedral_angle_d
X-RAY DIFFRACTIONx_dihedral_angle_d_na
X-RAY DIFFRACTIONx_dihedral_angle_d_prot
X-RAY DIFFRACTIONx_improper_angle_d
X-RAY DIFFRACTIONx_improper_angle_d_na
X-RAY DIFFRACTIONx_improper_angle_d_prot
X-RAY DIFFRACTIONx_mcbond_it
X-RAY DIFFRACTIONx_mcangle_it
X-RAY DIFFRACTIONx_scbond_it
X-RAY DIFFRACTIONx_scangle_it

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