Mass: 16761.391 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella enterica (bacteria) / Strain: enterica serovar Typhimurium / Gene: slyA, STM1444 / Plasmid: pMCSG19 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P40676
#2: DNA chain
DNA (5'-D(*AP*AP*TP*AP*AP*CP*TP*TP*AP*GP*CP*AP*AP*GP*CP*TP*AP*AP*TP*TP*AP*TP*A)-3')
Mass: 7055.625 Da / Num. of mol.: 1 / Source method: obtained synthetically
#3: DNA chain
DNA (5'-D(*TP*TP*AP*TP*AP*AP*TP*TP*AP*GP*CP*TP*TP*GP*CP*TP*AP*AP*GP*TP*TP*AP*T)-3')
Mass: 7059.592 Da / Num. of mol.: 1 / Source method: obtained synthetically
Sequence details
AUTHORS STATE THAT THE SEQUENCING EXPERIMENTS OF THE CLONES CONFIRMED THE CODING SEQUENCE AT THESE ...AUTHORS STATE THAT THE SEQUENCING EXPERIMENTS OF THE CLONES CONFIRMED THE CODING SEQUENCE AT THESE POSITIONS IS GAGCCG (CORRESPONDING TO GLU-PRO).
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.71 Å3/Da / Density % sol: 54.63 %
Redundancy: 14.9 % / Av σ(I) over netI: 35.32 / Number: 123915 / Rmerge(I) obs: 0.111 / Χ2: 2.02 / D res high: 4 Å / D res low: 50 Å / Num. obs: 8323 / % possible obs: 98.8
Diffraction reflection shell
Highest resolution (Å)
Lowest resolution (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
8.6
50
96.7
1
0.053
3.232
14.7
6.84
8.6
99
1
0.07
2.558
15.5
5.97
6.84
99.3
1
0.127
2.071
15.6
5.43
5.97
99.4
1
0.152
1.925
15.4
5.04
5.43
99.1
1
0.178
1.779
15.5
4.74
5.04
99
1
0.195
1.759
15.3
4.5
4.74
99
1
0.215
1.717
15
4.31
4.5
98.9
1
0.231
1.745
14.3
4.14
4.31
99.2
1
0.241
1.636
13.7
4
4.14
99
1
0.287
1.653
13.8
Reflection
Resolution: 2.96→50 Å / Num. obs: 11205 / % possible obs: 94.4 % / Redundancy: 12.5 % / Rmerge(I) obs: 0.081 / Χ2: 1.483 / Net I/σ(I): 10.4
Reflection shell
Resolution (Å)
Redundancy (%)
Rmerge(I) obs
Num. unique all
Χ2
Diffraction-ID
% possible all
2.96-3.08
7.7
0.26
705
1.169
1
63.2
3.08-3.2
8.6
0.147
958
1.35
1
83.1
3.2-3.34
10.5
0.147
1127
1.299
1
96.9
3.34-3.52
13.2
0.137
1156
1.46
1
99.5
3.52-3.74
14.1
0.146
1147
1.367
1
99.6
3.74-4.03
14.2
0.112
1169
1.424
1
99.7
4.03-4.44
14.1
0.091
1166
1.774
1
99.8
4.44-5.08
13.8
0.071
1198
1.417
1
100
5.08-6.39
13.5
0.068
1227
1.553
1
100
6.39-50
12.2
0.036
1352
1.676
1
99.1
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Phasing
Phasing
Method: MAD
Phasing set
ID
1
2
3
Phasing MAD
D res high: 5 Å / D res low: 1000 Å / FOM : 0.65 / Reflection: 2534
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