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Open data
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Basic information
Entry | Database: PDB / ID: 3ovw | |||||||||
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Title | ENDOGLUCANASE I NATIVE STRUCTURE | |||||||||
![]() | ENDOGLUCANASE I | |||||||||
![]() | HYDROLASE / GLYCOSYL HYDROLASE / ENDOGLUCANASE I / GLYCOSYLATED PROTEIN | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Davies, G.J. / Schulein, M. | |||||||||
![]() | ![]() Title: Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution. Authors: Sulzenbacher, G. / Schulein, M. / Davies, G.J. #1: ![]() Title: The Use of Conserved Cellulase Family-Specific Sequences to Clone Cellulase Homologue Cdnas from Fusarium Oxysporum Authors: Sheppard, P.O. / Grant, F.J. / Oort, P.J. / Sprecher, C.A. / Foster, D.C. / Hagen, F.S. / Upshall, A. / Mcknight, G.L. / O'Hara, P.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 172 KB | Display | ![]() |
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PDB format | ![]() | 135.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 407.8 KB | Display | ![]() |
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Full document | ![]() | 430.6 KB | Display | |
Data in XML | ![]() | 20.2 KB | Display | |
Data in CIF | ![]() | 31.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 44685.320 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Sugar | ChemComp-NAG / #3: Water | ChemComp-HOH / | Compound details | THIS STRUCTURE BELONGS TO FAMILY 7 OF GLYCOSYL HYDROLASES. THIS IS THE NATIVE ENZYME STRUCTURE. THE ...THIS STRUCTURE BELONGS TO FAMILY 7 OF GLYCOSYL HYDROLASES | Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48 % | ||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6.5 Details: 20 % PEG 8K, 0.2 M MAGNESIUM CHLORIDE, PH 6.5 FOR 0.1 M MOPS. METHOD: HANGING DROP VAPOR DIFFUSION, vapor diffusion - hanging drop | ||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Date: 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→15 Å / Num. obs: 32773 / % possible obs: 91 % / Redundancy: 2.4 % / Rmerge(I) obs: 0.072 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 1.87 % / Rmerge(I) obs: 0.185 / % possible all: 51.9 |
Reflection shell | *PLUS % possible obs: 51 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NATIVE STRUCTURE OF HUMICOLA INSOLENS EG I Resolution: 2.3→18 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.3→18 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection all: 32757 / Rfactor all: 0.158 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |