温度: 290 K / 手法: liquide diffusion in gel phase / pH: 10.5 詳細: SOLUTION A (0.1 ML): 50MM TRIS BUFFER, SATURATED WITH ACID URIC, 10% PEG 8000, PH 10.5, 0.5 MM AGAROSE IN CAPPILARY (HALF FILLING), SET AS A GEL (BY COOLING FROM 323 K TO ROOM TEMP). SOLUTION ...詳細: SOLUTION A (0.1 ML): 50MM TRIS BUFFER, SATURATED WITH ACID URIC, 10% PEG 8000, PH 10.5, 0.5 MM AGAROSE IN CAPPILARY (HALF FILLING), SET AS A GEL (BY COOLING FROM 323 K TO ROOM TEMP). SOLUTION B (0.1 ML): 20 MG/ML PROTEIN, SAME BUFFER, SET IN CONTACT WITHIN THE CAPILLARY WITH SOL.A. CRYSTALS DEVELOP IN THE GEL PHASE BY SLOW DIFFUSION. ALL SOLUTION DEGASED, CRYSTALLIZATIONS UNDER ARGON ATMOSPHERE (ANAEROBIC CONDITIONS), liquide diffusion in gel phase, temperature 290K