ソフトウェア | 名称 | バージョン | 分類 | NB |
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DENZO | | データ削減 | | SCALEPACK | | データスケーリング | | REFMAC | refmac_5.5.0054精密化 | | PDB_EXTRACT | 3.004 | データ抽出 | | SBC-Collect | | データ収集 | | HKL-3000 | | データ削減 | | HKL-3000 | | データスケーリング | | SHELX | | 位相決定 | | MLPHARE | | 位相決定 | | 直接法 | | 位相決定 | | ARP/wARP | | モデル構築 | | Coot | | モデル構築 | | |
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精密化 | 解像度: 2→33.17 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.948 / WRfactor Rfree: 0.244 / WRfactor Rwork: 0.192 / SU B: 7.896 / SU ML: 0.098 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.141 / ESU R Free: 0.134 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : RESIDUAL ONLY
| Rfactor | 反射数 | %反射 | Selection details |
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Rfree | 0.217 | 1311 | 5.1 % | RANDOM |
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Rwork | 0.182 | - | - | - |
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obs | 0.184 | 25753 | 99.56 % | - |
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK |
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原子変位パラメータ | Biso mean: 15.043 Å2
| Baniso -1 | Baniso -2 | Baniso -3 |
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1- | -0.1 Å2 | -0.05 Å2 | 0 Å2 |
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2- | - | -0.1 Å2 | 0 Å2 |
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3- | - | - | 0.15 Å2 |
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精密化ステップ | サイクル: LAST / 解像度: 2→33.17 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 2122 | 0 | 5 | 89 | 2216 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | Dev ideal target | 数 |
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X-RAY DIFFRACTION | r_bond_refined_d0.011 | 0.022 | 2181 | X-RAY DIFFRACTION | r_angle_refined_deg1.151 | 1.985 | 2943 | X-RAY DIFFRACTION | r_dihedral_angle_1_deg5.341 | 5 | 272 | X-RAY DIFFRACTION | r_dihedral_angle_2_deg35.833 | 26.222 | 90 | X-RAY DIFFRACTION | r_dihedral_angle_3_deg14.675 | 15 | 401 | X-RAY DIFFRACTION | r_dihedral_angle_4_deg5.954 | 15 | 2 | X-RAY DIFFRACTION | r_chiral_restr0.083 | 0.2 | 344 | X-RAY DIFFRACTION | r_gen_planes_refined0.004 | 0.021 | 1583 | X-RAY DIFFRACTION | r_mcbond_it0.471 | 1.5 | 1372 | X-RAY DIFFRACTION | r_mcangle_it0.864 | 2 | 2206 | X-RAY DIFFRACTION | r_scbond_it1.457 | 3 | 809 | X-RAY DIFFRACTION | r_scangle_it2.281 | 4.5 | 737 | | | | | | | | | | | | |
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20 解像度 (Å) | Rfactor Rfree | Num. reflection Rfree | Rfactor Rwork | Num. reflection Rwork | Num. reflection all | % reflection obs (%) |
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2.4-2.462 | 0.322 | 177 | 0.264 | 3514 | 3700 | 99.757 | 2.462-2.53 | 0.375 | 177 | 0.265 | 3439 | 3630 | 99.614 | 2.53-2.603 | 0.32 | 172 | 0.256 | 3354 | 3537 | 99.689 | 2.603-2.682 | 0.341 | 176 | 0.265 | 3237 | 3426 | 99.621 | 2.682-2.77 | 0.311 | 156 | 0.262 | 3149 | 3321 | 99.518 | 2.77-2.867 | 0.313 | 187 | 0.253 | 3030 | 3231 | 99.567 | 2.867-2.975 | 0.329 | 145 | 0.25 | 2939 | 3105 | 99.324 | 2.975-3.096 | 0.348 | 159 | 0.257 | 2841 | 3016 | 99.469 | 3.096-3.233 | 0.319 | 148 | 0.235 | 2697 | 2869 | 99.163 | 3.233-3.39 | 0.268 | 129 | 0.207 | 2615 | 2773 | 98.954 | 3.39-3.572 | 0.211 | 137 | 0.185 | 2443 | 2631 | 98.062 | 3.572-3.788 | 0.25 | 126 | 0.183 | 2307 | 2496 | 97.476 | 3.788-4.048 | 0.216 | 112 | 0.154 | 2181 | 2362 | 97.079 | 4.048-4.369 | 0.189 | 108 | 0.148 | 2018 | 2187 | 97.211 | 4.369-4.782 | 0.215 | 109 | 0.142 | 1876 | 2042 | 97.209 | 4.782-5.34 | 0.184 | 85 | 0.164 | 1742 | 1877 | 97.336 | 5.34-6.154 | 0.239 | 95 | 0.186 | 1493 | 1637 | 97.007 | 6.154-7.507 | 0.233 | 83 | 0.194 | 1277 | 1415 | 96.113 | 7.507-10.49 | 0.166 | 46 | 0.156 | 1039 | 1129 | 96.103 | 10.49-48.337 | 0.216 | 33 | 0.22 | 589 | 695 | 89.496 |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 14.6001 | 10.3781 | -0.5002 | 21.9335 | 4.0835 | 17.5715 | 0.1744 | -0.2938 | 0.1725 | 0.1169 | -0.1347 | -1.3008 | -0.8542 | 1.3128 | -0.0397 | 0.2348 | -0.0223 | 0.0263 | 0.2365 | 0.0404 | 0.2207 | 5.2345 | 24.9102 | 57.8079 | 2 | 8.5059 | 8.8538 | 10.0781 | 9.1991 | 13.3159 | 22.6979 | -0.0699 | -0.2366 | 0.5126 | -0.0397 | -0.6348 | 0.5675 | 0.3032 | -1.3628 | 0.7048 | 0.3095 | 0.07 | -0.0484 | 0.2836 | -0.06 | 0.3291 | -7.3747 | 19.7489 | 49.1679 | 3 | 9.2245 | 6.7804 | -0.5017 | 15.4329 | 5.7879 | 5.3613 | -0.0196 | 0.3653 | -0.0441 | -0.8617 | -0.2159 | 0.1323 | -0.5648 | -0.5542 | 0.2355 | 0.2962 | 0.0926 | -0.0408 | 0.213 | 0.0267 | 0.1033 | -10.26 | 27.9402 | 48.7022 | 4 | 14.2763 | 11.3597 | 2.1409 | 25.831 | 17.3193 | 14.7562 | -0.0591 | 0.4999 | 0.7442 | -0.0842 | -0.3472 | -0.1469 | -0.4666 | 0.6428 | 0.4063 | 0.3139 | -0.0245 | -0.0084 | 0.3216 | 0.1509 | 0.3142 | -1.6923 | 33.2403 | 52.7533 | 5 | 13.1935 | -7.364 | 4.2273 | 34.1883 | -5.4626 | -4.6748 | -0.5066 | -0.301 | 1.3466 | 0.2646 | 0.3569 | -0.4657 | -0.1426 | 0.1109 | 0.1497 | 0.5394 | -0.101 | 0.0707 | 0.5035 | -0.0128 | 0.504 | 7.4792 | 31.8382 | 54.4302 | 6 | 10.0406 | 11.1999 | 2.9662 | 21.9365 | 2.5069 | 5.888 | -0.1165 | 0.2876 | -0.0371 | -0.5683 | 0.2386 | 0.1541 | 0.4057 | 0.0673 | -0.1221 | 0.2325 | 0.045 | 0.0279 | 0.1591 | 0.0421 | 0.1192 | -1.8251 | 15.7227 | 50.1019 | 7 | 17.7415 | 10.3308 | -7.1245 | 18.5114 | -5.5068 | 1.2693 | -0.0355 | 0.443 | 0.1848 | 0.3411 | 0.1343 | -0.2892 | -0.0885 | 0.0217 | -0.0989 | 0.3341 | -0.0442 | 0.0119 | 0.2708 | 0.0268 | 0.2666 | -12.3888 | 0.8343 | 48.3685 | 8 | 6.362 | 7.5134 | 3.8907 | 6.4076 | -2.4856 | 19.982 | -0.5322 | 0.4769 | 0.6343 | -0.576 | 0.684 | 0.9864 | -0.7583 | -1.0507 | -0.1517 | 0.5198 | -0.0231 | -0.1199 | 0.5436 | 0.1356 | 0.319 | -19.9339 | -3.456 | 40.0005 | 9 | 3.8227 | 3.5396 | -2.5301 | 14.6416 | 0.7245 | -0.2437 | -0.1091 | 0.9842 | -0.4262 | -0.5591 | 0.2627 | -0.0122 | -0.0626 | -0.4104 | -0.1535 | 0.8205 | -0.0699 | -0.0072 | 0.6408 | -0.0078 | 0.4592 | -10.7374 | 0.3306 | 36.8706 | 10 | 25.532 | 23.2095 | -2.8346 | 76.8914 | 1.7078 | 22.7746 | -0.4711 | -0.5546 | -0.491 | -0.4117 | 0.3422 | 0.5451 | 1.9763 | 0.1525 | 0.1288 | 0.4146 | 0.0346 | 0.0196 | 0.2117 | 0.0615 | 0.2256 | -7.634 | 8.7741 | 39.8615 | 11 | 17.8784 | -0.069 | 1.2666 | 23.2145 | 0.0638 | 8.6851 | 0.1879 | 0.2089 | -0.497 | -0.4885 | 0.0984 | 0.3455 | -0.1113 | 0.4281 | -0.2863 | 0.337 | 0.0705 | 0.0144 | 0.2121 | 0.0301 | 0.0745 | -3.3766 | 15.8683 | 40.068 | 12 | 2.5429 | 1.1443 | 5.0193 | 14.7718 | 8.4058 | 7.4046 | 0.0035 | 0.1852 | 0.3233 | -0.8225 | -0.1668 | -0.102 | -0.4671 | 0.0438 | 0.1633 | 0.4174 | 0.0383 | 0.054 | 0.2556 | 0.1113 | 0.2123 | -1.4409 | 25.2822 | 42.6262 | 13 | 2.3694 | -2.8174 | 1.1597 | 21.9577 | -9.6479 | 19.3862 | -0.1583 | 0.5118 | 0.4999 | -0.5775 | -0.2371 | -1.2874 | -0.8344 | 0.8208 | 0.3954 | 0.3404 | -0.1057 | 0.0838 | 0.3109 | 0.0567 | 0.3171 | 5.2643 | 26.3007 | 45.1506 | 14 | 12.8137 | 10.9794 | -6.7024 | 15.74 | -6.8548 | 7.9776 | 0.0613 | 0.1481 | -0.7314 | -0.1463 | 0.0431 | 0.0755 | 0.5865 | -0.1199 | -0.1044 | 0.3238 | 0.0455 | -0.0419 | 0.1636 | 0.0219 | 0.2182 | -2.7223 | 9.6317 | 51.5854 | 15 | 22.5444 | 11.0655 | 6.7718 | 16.0458 | 6.493 | 12.1972 | 0.059 | -0.6258 | -1.2724 | 0.497 | -0.1672 | -0.1189 | 0.9128 | 0.0254 | 0.1082 | 0.3314 | 0.0466 | 0.0119 | 0.1039 | 0.0716 | 0.2208 | -2.4573 | 12.0396 | 60.3601 | 16 | 13.8639 | -1.5139 | 0.9077 | 1.4209 | 0.5112 | 5.3571 | 0.1671 | 0.0184 | -0.6182 | 0.0925 | -0.0763 | 0.0599 | 0.3995 | -0.0349 | -0.0908 | 0.2134 | -0.0147 | -0.0002 | 0.0916 | 0.0202 | 0.1416 | -4.4346 | 21.5748 | 57.8844 | 17 | 27.9413 | -2.8233 | -14.0775 | 21.4742 | -3.8521 | 12.3874 | 0.2547 | -0.2504 | -0.5609 | -0.522 | -0.4377 | -1.1244 | 1.0474 | -0.103 | 0.183 | 0.465 | -0.1202 | -0.0108 | 0.3066 | -0.0591 | 0.4856 | -15.0781 | 15.4587 | 54.2444 | 18 | 9.7369 | -2.4929 | -1.8489 | 3.7648 | 0.3465 | 2.4179 | -0.076 | 0.1247 | -0.4694 | 0.2567 | -0.1854 | 0.5036 | 0.3929 | -0.2746 | 0.2614 | 0.3045 | -0.0647 | 0.0754 | 0.2097 | -0.0061 | 0.2392 | -13.7048 | 19.4459 | 65.4314 | 19 | 11.991 | -7.6965 | 3.4112 | 7.8271 | -0.5312 | 9.5604 | 0.2691 | 0.3365 | -0.1901 | -0.2129 | -0.2545 | 0.4509 | 0.0787 | -0.3326 | -0.0146 | 0.1792 | -0.0293 | 0.0375 | 0.1306 | 0.0284 | 0.1458 | -8.5203 | 25.1843 | 60.3405 | 20 | 6.3784 | 0.4674 | -0.63 | 8.2079 | -2.0894 | 16.3577 | 0.1076 | 0.0871 | 0.2173 | 0.1823 | -0.027 | -0.0186 | -0.2157 | 0.3129 | -0.0805 | 0.136 | 0.0225 | 0.02 | 0.1575 | -0.0374 | 0.201 | -7.1308 | 38.3558 | 67.8903 | 21 | 19.0091 | 6.3085 | -13.3866 | 15.9691 | -2.819 | 20.3758 | -0.0346 | -1.2389 | -0.3295 | 1.1309 | -0.3191 | 0.1694 | 0.2183 | 0.3344 | 0.3537 | 0.3073 | 0.0234 | -0.0199 | 0.222 | 0.0452 | 0.1587 | -3.2396 | 28.8855 | 77.3095 | 22 | 0.3143 | -0.3788 | -4.4751 | 1.036 | -2.9584 | 19.6637 | 0.0143 | 0.1331 | 0.2454 | 0.2055 | -0.0829 | -0.2036 | 0.0479 | 0.6805 | 0.0687 | 0.2108 | 0.073 | -0.0133 | 0.3225 | -0.0098 | 0.2391 | 1.2964 | 32.785 | 69.107 | 23 | 7.9144 | -5.2484 | -2.853 | 24.2803 | -0.0576 | 6.9141 | -0.1682 | 0.3621 | 0.5154 | 0.0394 | -0.076 | -1.0249 | -0.4546 | 0.362 | 0.2442 | 0.2552 | -0.0097 | 0.0454 | 0.3697 | 0.0111 | 0.2559 | 4.4588 | 35.3943 | 63.4742 | 24 | 17.933 | 4.7189 | -5.4312 | 7.9895 | 1.0747 | 14.5883 | 0.0142 | 1.4065 | 0.2241 | -1.5935 | 0.3826 | -1.0147 | -0.3942 | 1.089 | -0.3967 | 0.4982 | -0.0909 | 0.2112 | 0.5054 | -0.0097 | 0.5287 | -0.0013 | 42.7951 | 60.8136 | 25 | 17.4977 | 2.8744 | -5.9455 | 5.8923 | 1.0837 | 7.658 | 0.2465 | -0.7051 | -0.2344 | 0.6933 | -0.3944 | 0.5658 | -0.2563 | -0.2959 | 0.1479 | 0.1892 | 0.0042 | 0.0659 | 0.158 | -0.0278 | 0.2236 | -13.7589 | 41.1174 | 75.2045 | 26 | 33.4306 | 18.5938 | 6.6496 | 16.0072 | 7.6268 | 14.2128 | -0.4146 | 0.5369 | 0.62 | -0.1383 | 0.7742 | -0.3979 | 0.0942 | 1.3913 | -0.3596 | 0.2566 | 0.1095 | 0.0476 | 0.4429 | -0.1002 | 0.4269 | -32.0199 | 38.6735 | 91.5502 | 27 | 38.0541 | 2.93 | -24.1924 | 20.1402 | -6.278 | 35.432 | 0.1692 | -0.8348 | 0.1896 | 0.651 | 0.0026 | 0.628 | 0.2384 | -1.2087 | -0.1718 | 0.3978 | -0.1348 | 0.0788 | 0.6414 | -0.157 | 0.3412 | -19.8013 | 43.8151 | 89.4579 | 28 | 39.0876 | -3.6198 | -20.6623 | 1.368 | -1.5998 | 19.5398 | 0.4578 | -1.4011 | 0.3593 | 0.6261 | -0.0503 | 0.6034 | -0.4393 | 0.4546 | -0.4076 | 0.5348 | -0.1883 | 0.1979 | 0.3661 | -0.2091 | 0.475 | -7.2028 | 42.9302 | 82.0184 | 29 | 21.8899 | 2.787 | -10.5625 | 0.553 | -2.2942 | 9.5498 | 0.3015 | -0.49 | 0.3767 | 0.1877 | -0.2818 | 0.0932 | 0.3336 | -0.0061 | -0.0197 | 0.4298 | -0.1942 | -0.0663 | 0.3076 | 0.0169 | 0.2015 | 0.2129 | 40.2289 | 75.4803 | 30 | 15.0076 | -4.0659 | -1.6793 | 2.8108 | -0.991 | 2.8523 | -0.0746 | -0.1399 | 0.1172 | 0.1142 | -0.087 | -0.1076 | 0.1211 | 0.137 | 0.1616 | 0.1898 | -0.0439 | 0.002 | 0.1688 | -0.0094 | 0.1634 | -0.1254 | 44.823 | 71.3221 | 31 | 13.9459 | 10.5304 | -5.4808 | 12.7367 | -3.3271 | 5.4299 | 0.2694 | 0.0849 | 0.5056 | 0.2136 | -0.2406 | 0.8819 | -0.1851 | -0.4733 | -0.0288 | 0.2056 | 0.0243 | 0.0498 | 0.3086 | -0.0853 | 0.3451 | -19.6673 | 41.5445 | 75.1842 | 32 | 3.269 | -3.7317 | -3.4134 | 7.836 | 2.0768 | 25.0843 | -0.0428 | 0.3379 | -0.0765 | -0.2358 | -0.2747 | 1.1309 | 0.2279 | -1.0868 | 0.3175 | 0.1317 | -0.0215 | 0.0353 | 0.2689 | -0.0844 | 0.3883 | -20.5044 | 37.2774 | 68.5985 | 33 | 23.4191 | 4.8158 | -7.9662 | 8.1776 | -2.4419 | 20.911 | -0.1197 | 0.2929 | 1.0156 | -0.2799 | -0.1322 | 0.8085 | -0.8168 | -1.1151 | 0.2518 | 0.2037 | 0.0849 | -0.0397 | 0.1891 | -0.0052 | 0.2572 | -15.2035 | 41.6658 | 62.4117 | 34 | 7.8983 | -2.526 | 4.8321 | 5.4079 | 0.0968 | 7.7869 | -0.0596 | -0.0551 | -0.22 | 0.093 | -0.3219 | 0.0537 | 0.0727 | -0.1131 | 0.3815 | 0.1299 | -0.0315 | 0.0286 | 0.1176 | -0.0159 | 0.1512 | -9.1769 | 33.8384 | 64.705 | 35 | 27.6239 | -3.5618 | -3.8983 | -0.6366 | 1.1137 | 0.9411 | -0.0463 | -0.0975 | 0.0036 | 0.3842 | -0.1802 | 0.0241 | 0.3487 | -0.2194 | 0.2265 | 0.4258 | -0.0605 | 0.078 | 0.2621 | 0.0382 | 0.2449 | -13.5744 | 27.1541 | 76.4581 | 36 | 11.3598 | 3.1583 | -0.7955 | 9.0751 | 2.2745 | 26.6317 | 0.1947 | -0.8992 | 0.223 | 0.5653 | -0.25 | 0.8047 | 0.7108 | -1.191 | 0.0553 | 0.2536 | -0.1239 | 0.1756 | 0.2943 | -0.1016 | 0.3374 | -22.6986 | 27.9526 | 75.8567 | 37 | 10.9079 | 5.2304 | -2.7776 | 27.4699 | -9.4948 | 18.4563 | -0.2124 | 0.5456 | 0.0298 | -0.4408 | 0.2576 | 1.7794 | 0.7283 | -1.1798 | -0.0452 | 0.1172 | -0.0453 | 0.003 | 0.2879 | -0.1066 | 0.348 | -20.6662 | 27.7589 | 63.7734 | 38 | 4.6289 | -3.6223 | 3.1456 | 10.3297 | -0.8246 | 4.6568 | -0.0642 | -0.101 | -0.2478 | 0.3298 | 0.0724 | 0.3939 | 0.0464 | -0.1906 | -0.0082 | 0.1336 | -0.0207 | 0.0597 | 0.1471 | 0.0022 | 0.1826 | -9.3485 | 28.5134 | 65.0411 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Auth asym-ID | Auth seq-ID |
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1 | X-RAY DIFFRACTION | 1 | A1 - 5 | 2 | X-RAY DIFFRACTION | 2 | A6 - 10 | 3 | X-RAY DIFFRACTION | 3 | A11 - 20 | 4 | X-RAY DIFFRACTION | 4 | A21 - 25 | 5 | X-RAY DIFFRACTION | 5 | A26 - 32 | 6 | X-RAY DIFFRACTION | 6 | A33 - 39 | 7 | X-RAY DIFFRACTION | 7 | A40 - 44 | 8 | X-RAY DIFFRACTION | 8 | A45 - 52 | 9 | X-RAY DIFFRACTION | 9 | A53 - 58 | 10 | X-RAY DIFFRACTION | 10 | A59 - 63 | 11 | X-RAY DIFFRACTION | 11 | A64 - 69 | 12 | X-RAY DIFFRACTION | 12 | A70 - 76 | 13 | X-RAY DIFFRACTION | 13 | A77 - 83 | 14 | X-RAY DIFFRACTION | 14 | A84 - 91 | 15 | X-RAY DIFFRACTION | 15 | A92 - 100 | 16 | X-RAY DIFFRACTION | 16 | A101 - 111 | 17 | X-RAY DIFFRACTION | 17 | A112 - 117 | 18 | X-RAY DIFFRACTION | 18 | A118 - 130 | 19 | X-RAY DIFFRACTION | 19 | A131 - 138 | 20 | X-RAY DIFFRACTION | 20 | B1 - 10 | 21 | X-RAY DIFFRACTION | 21 | B11 - 16 | 22 | X-RAY DIFFRACTION | 22 | B17 - 22 | 23 | X-RAY DIFFRACTION | 23 | B23 - 27 | 24 | X-RAY DIFFRACTION | 24 | B28 - 33 | 25 | X-RAY DIFFRACTION | 25 | B34 - 40 | 26 | X-RAY DIFFRACTION | 26 | B44 - 51 | 27 | X-RAY DIFFRACTION | 27 | B52 - 61 | 28 | X-RAY DIFFRACTION | 28 | B62 - 70 | 29 | X-RAY DIFFRACTION | 29 | B71 - 75 | 30 | X-RAY DIFFRACTION | 30 | B76 - 85 | 31 | X-RAY DIFFRACTION | 31 | B86 - 91 | 32 | X-RAY DIFFRACTION | 32 | B92 - 97 | 33 | X-RAY DIFFRACTION | 33 | B98 - 102 | 34 | X-RAY DIFFRACTION | 34 | B103 - 108 | 35 | X-RAY DIFFRACTION | 35 | B109 - 116 | 36 | X-RAY DIFFRACTION | 36 | B117 - 123 | 37 | X-RAY DIFFRACTION | 37 | B124 - 130 | 38 | X-RAY DIFFRACTION | 38 | B131 - 138 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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