endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding / cytoplasm 類似検索 - 分子機能
Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) ...Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
解像度: 2→2.1 Å / 冗長度: 7.42 % / Rmerge(I) obs: 0.172 / Mean I/σ(I) obs: 5.55 / Num. unique all: 1280 / Rsym value: 0.2818 / % possible all: 100
-
位相決定
位相決定
手法: 分子置換
Phasing MR
Rfactor: 34.55 / Model details: Phaser MODE: MR_AUTO
最高解像度
最低解像度
Rotation
2.5 Å
37.34 Å
Translation
2.5 Å
37.34 Å
-
解析
ソフトウェア
名称
バージョン
分類
NB
SAINT
データスケーリング
PHASER
2.1.3
位相決定
REFMAC
精密化
PDB_EXTRACT
3.005
データ抽出
APEX
データ収集
SAINT
データ削減
精密化
構造決定の手法: 分子置換 開始モデル: 3BDC (SNase D+PHS), with residues 66 and 113-116 truncated to Ala, all waters, Ca2+ and THP removed, and all b-factors truncated to 20.00A^2