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Yorodumi- PDB-3fgz: Crystal Structure of CheY triple mutant F14E, N59M, E89R complexe... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3fgz | |||||||||
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Title | Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+ | |||||||||
Components | Chemotaxis protein cheY | |||||||||
Keywords | SIGNALING PROTEIN / Response Regulator / Receiver Domain / BeF3 / Two-Component Signal Transduction / Chemotaxis / Flagellar rotation / Magnesium / Metal-binding / Phosphoprotein / Two-component regulatory system | |||||||||
Function / homology | Function and homology information bacterial-type flagellum basal body, C ring / bacterial-type flagellum rotor complex / bacterial-type flagellum-dependent swimming motility / regulation of bacterial-type flagellum-dependent cell motility / aerotaxis / bacterial-type flagellum / regulation of chemotaxis / thermotaxis / internal peptidyl-lysine acetylation / phosphorelay response regulator activity ...bacterial-type flagellum basal body, C ring / bacterial-type flagellum rotor complex / bacterial-type flagellum-dependent swimming motility / regulation of bacterial-type flagellum-dependent cell motility / aerotaxis / bacterial-type flagellum / regulation of chemotaxis / thermotaxis / internal peptidyl-lysine acetylation / phosphorelay response regulator activity / acetyltransferase activity / phosphorelay signal transduction system / chemotaxis / magnesium ion binding / signal transduction / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | |||||||||
Authors | Wollish, A.C. / Miller, P.J. / Pazy, Y. / Collins, E.J. / Bourret, R.B. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2009 Title: Matching Biochemical Reaction Kinetics to the Timescales of Life: Structural Determinants That Influence the Autodephosphorylation Rate of Response Regulator Proteins. Authors: Pazy, Y. / Wollish, A.C. / Thomas, S.A. / Miller, P.J. / Collins, E.J. / Bourret, R.B. / Silversmith, R.E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3fgz.cif.gz | 67.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3fgz.ent.gz | 48.7 KB | Display | PDB format |
PDBx/mmJSON format | 3fgz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3fgz_validation.pdf.gz | 454.4 KB | Display | wwPDB validaton report |
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Full document | 3fgz_full_validation.pdf.gz | 455.1 KB | Display | |
Data in XML | 3fgz_validation.xml.gz | 13.4 KB | Display | |
Data in CIF | 3fgz_validation.cif.gz | 18.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fg/3fgz ftp://data.pdbj.org/pub/pdb/validation_reports/fg/3fgz | HTTPS FTP |
-Related structure data
Related structure data | 3f7nC 3fftC 3ffwC 3ffxC 1fqwS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 14008.249 Da / Num. of mol.: 2 / Mutation: F14E, N59M, E89R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K-12 / Gene: b1882, cheY, JW1871 / Production host: Escherichia coli (E. coli) / Strain (production host): KO641recA / References: UniProt: P0AE67 |
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-Non-polymers , 5 types, 176 molecules
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-NH4 / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.14 Å3/Da / Density % sol: 70.26 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.4 Details: Tris buffer, Ammonium Sulfate, Glycerol, BeCl2, NaF, MnCl2, pH 8.4, vapor diffusion, hanging drop, temperature 298K |
-Data collection
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Sep 19, 2006 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2→80.85 Å / Num. obs: 31410 / % possible obs: 97.2 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.065 / Χ2: 1.086 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1FQW Resolution: 2→80.85 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.934 / SU ML: 0.078 / ESU R: 0.129 / ESU R Free: 0.122
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Displacement parameters | Biso mean: 25.411 Å2
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Refinement step | Cycle: LAST / Resolution: 2→80.85 Å
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