[English] 日本語
Yorodumi- PDB-3eyb: Structural and functional insights into the ligand binding domain... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3eyb | ||||||
---|---|---|---|---|---|---|---|
Title | Structural and functional insights into the ligand binding domain of a non-duplicated RXR from the invertebrate chordate amphioxus | ||||||
Components | Nuclear hormone receptor RXR | ||||||
Keywords | TRANSCRIPTION / Amphioxus / Retinoid X receptor / nuclear receptor / ligand binding / DNA-binding / Metal-binding / Nucleus / Receptor / Transcription regulation / Zinc-finger / Structural Genomics / Structural Proteomics in Europe / SPINE | ||||||
Function / homology | Function and homology information nuclear steroid receptor activity / sequence-specific DNA binding / DNA-binding transcription factor activity / zinc ion binding / nucleus Similarity search - Function | ||||||
Biological species | Branchiostoma floridae (Florida lancelet) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.79 Å | ||||||
Authors | Tocchini-Valentini, G.D. / Rochel, N. / Moras, D. / Structural Proteomics in Europe (SPINE) | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2009 Title: Structural and functional insights into the ligand-binding domain of a nonduplicated retinoid X nuclear receptor from the invertebrate chordate amphioxus Authors: Tocchini-Valentini, G.D. / Rochel, N. / Escriva, H. / Germain, P. / Peluso-Iltis, C. / Paris, M. / Sanglier-Cianferani, S. / Van Dorsselaer, A. / Moras, D. / Laudet, V. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3eyb.cif.gz | 158 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3eyb.ent.gz | 125.7 KB | Display | PDB format |
PDBx/mmJSON format | 3eyb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/3eyb ftp://data.pdbj.org/pub/pdb/validation_reports/ey/3eyb | HTTPS FTP |
---|
-Related structure data
Related structure data | 1lbd S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 24697.746 Da / Num. of mol.: 4 / Fragment: UNP residues 266-484, Ligand binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Branchiostoma floridae (Florida lancelet) Plasmid: pET15 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE3 / References: UniProt: Q8MX78 #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.09 % |
---|---|
Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 1.5 M Na acetate, 0.1 M ADA pH=6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 200 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 |
Detector | Detector: CCD / Date: May 1, 2004 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.79→15 Å / Num. all: 22831 / Num. obs: 22831 / % possible obs: 90.34 % / Observed criterion σ(F): 2 / Redundancy: 4.5 % / Rmerge(I) obs: 0.082 / Rsym value: 0.082 / Net I/σ(I): 5.4 |
Reflection shell | Resolution: 2.79→2.89 Å / Mean I/σ(I) obs: 2.5 / Rsym value: 0.2 / % possible all: 75 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1LBD 1lbd Resolution: 2.79→15 Å / Isotropic thermal model: isotropic / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Displacement parameters | Biso mean: 56.5 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.79→15 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.79→2.86 Å / Num. reflection obs: 1339 |