- PDB-3ef0: The Structure of Fcp1, an essential RNA polymerase II CTD phosphatase -
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Open data
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Basic information
Entry
Database: PDB / ID: 3ef0
Title
The Structure of Fcp1, an essential RNA polymerase II CTD phosphatase
Components
RNA polymerase II subunit A C-terminal domain phosphatase
Keywords
HYDROLASE / phosphatase / CTD / FCPH / BRCT / AlF4 / transition state analog / Cobalt / Magnesium / Manganese / Metal-binding / Nucleus / Protein phosphatase
Function / homology
Function and homology information
Formation of the Early Elongation Complex / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / RNA polymerase II CTD heptapeptide repeat phosphatase activity / signaling / myosin phosphatase activity / protein-serine/threonine phosphatase / nucleus / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 175 / Source method: isolated from a natural source / Formula: H2O
Sequence details
UNP Q9P376 RESIDUES 330-393 ARE DELETED AND REPLACED BY RESIDUES 'SG'
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.35 Å3/Da / Density % sol: 47.56 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Fcp1-AlF4--Mg was obtained by incubating 250 M Fcp1(149-580)- 330-393 with 500 M AlCl3, 5 mM NaF and 5 mM MgCl2 on ice for 2 h prior to crystallization by hanging drop vapor diffusion ...Details: Fcp1-AlF4--Mg was obtained by incubating 250 M Fcp1(149-580)- 330-393 with 500 M AlCl3, 5 mM NaF and 5 mM MgCl2 on ice for 2 h prior to crystallization by hanging drop vapor diffusion against 20% PEG-3350, 190 mM ammonium formate (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K
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