+Open data
-Basic information
Entry | Database: PDB / ID: 3dvo | ||||||
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Title | SgrAI with cognate DNA and calcium bound | ||||||
Components |
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Keywords | HYDROLASE/DNA / restriction enzyme-DNA complex / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptomyces griseus (bacteria) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.892 Å | ||||||
Authors | Dunten, P.W. / Horton, N.C. / Little, E.J. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2008 Title: The structure of SgrAI bound to DNA; recognition of an 8 base pair target. Authors: Dunten, P.W. / Little, E.J. / Gregory, M.T. / Manohar, V.M. / Dalton, M. / Hough, D. / Bitinaite, J. / Horton, N.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3dvo.cif.gz | 325.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3dvo.ent.gz | 257.3 KB | Display | PDB format |
PDBx/mmJSON format | 3dvo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dv/3dvo ftp://data.pdbj.org/pub/pdb/validation_reports/dv/3dvo | HTTPS FTP |
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-Related structure data
Related structure data | 3dpgSC 3dw9C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 5517.567 Da / Num. of mol.: 4 / Source method: obtained synthetically / Details: includes the SgrAI recognition sequence / Source: (synth.) synthetic construct (others) #2: Protein | Mass: 37941.906 Da / Num. of mol.: 4 / Mutation: N63D Source method: isolated from a genetically manipulated source Details: coexpressed with MspI methyltransferase from pBAKMspIM Source: (gene. exp.) Streptomyces griseus (bacteria) / Gene: sgraIR / Plasmid: pET21a_SgrAIR / Production host: Escherichia coli (E. coli) / Strain (production host): ER2566 References: UniProt: Q9F6L0, type II site-specific deoxyribonuclease #3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.78 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop Details: PEG4000, buffer, NaCl, CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K | ||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 1.89 Å / Num. obs: 126080 / % possible obs: 92.8 % / Redundancy: 3.4 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 6.5 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine) / Classification: refinement | ||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 3dpg Resolution: 1.892→41.068 Å / SU ML: 0.36 / Phase error: 28.53 / Stereochemistry target values: Engh & Huber
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 49.445 Å2 / ksol: 0.401 e/Å3 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.892→41.068 Å
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Refine LS restraints |
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