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Yorodumi- PDB-3cts: CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF TWO DIFFERENT FO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3cts | ||||||
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Title | CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF TWO DIFFERENT FORMS OF CITRATE SYNTHASE AT 2.7 AND 1.7 ANGSTROMS RESOLUTION | ||||||
Components | CITRATE SYNTHASE | ||||||
Keywords | OXO-ACID-LYASE | ||||||
Function / homology | CITRIC ACID / COENZYME A Function and homology information | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.7 Å | ||||||
Authors | Remington, S. / Wiegand, G. / Huber, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1982 Title: Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution. Authors: Remington, S. / Wiegand, G. / Huber, R. #1: Journal: Eur.J.Biochem. / Year: 1979 Title: Crystal Structure Analysis of the Tetragonal Crystal Form and Preliminary Molecular Model of Pig-Heart Citrate Synthase Authors: Wiegand, G. / Kukla, D. / Scholze, H. / Jones, T.A. / Huber, R. #2: Journal: Proc.Natl.Acad.Sci.USA / Year: 1981 Title: Primary Structure of Porcine Heart Citrate Synthase Authors: Bloxham, D.P. / Parmelee, D.C. / Kumar, S. / Wade, R.D. / Ericsson, L.H. / Neurath, H. / Walsh, K.A. / Titani, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3cts.cif.gz | 82.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3cts.ent.gz | 67.5 KB | Display | PDB format |
PDBx/mmJSON format | 3cts.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3cts_validation.pdf.gz | 424.1 KB | Display | wwPDB validaton report |
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Full document | 3cts_full_validation.pdf.gz | 427 KB | Display | |
Data in XML | 3cts_validation.xml.gz | 5.2 KB | Display | |
Data in CIF | 3cts_validation.cif.gz | 9.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ct/3cts ftp://data.pdbj.org/pub/pdb/validation_reports/ct/3cts | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: AN OCCUPANCY OF 0.0 INDICATES THAT THE ATOM COULD NOT BE FOUND IN THE ELECTRON DENSITY MAP AND IS INCLUDED AS A DUMMY ATOM. 2: RESIDUES 83, 292 AND 434 CONSIST OF ONLY ONE ATOM, N. NONE OF THESE ARE EXPLICIT DISCONTINUITIES, BUT MERELY CORRESPOND TO POORLY DEFINED REGIONS WHICH HAVE BEEN OMITTED. |
-Components
#1: Protein | Mass: 37208.820 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Tissue: HEART MUSCLE / References: EC: 4.1.3.7 |
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#2: Chemical | ChemComp-COA / |
#3: Chemical | ChemComp-CIT / |
#4: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE OF CHICKEN HEART CITRATE SYNTHASE HAS NOT BEEN DETERMINED. IT IS EXPECTED TO BE HIGHLY ...THE SEQUENCE OF CHICKEN HEART CITRATE SYNTHASE HAS NOT BEEN DETERMINED |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.13 Å3/Da / Density % sol: 60.74 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS |
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-Processing
Software | Name: EREF / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.7→5 Å / Rfactor Rwork: 0.192 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→5 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.192 / Highest resolution: 1.7 Å / Lowest resolution: 5 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: o_angle_d / Dev ideal: 2.9 |