+Open data
-Basic information
Entry | Database: PDB / ID: 3c9c | ||||||
---|---|---|---|---|---|---|---|
Title | Structural Basis of Histone H4 Recognition by p55 | ||||||
Components |
| ||||||
Keywords | TRANSCRIPTION REPRESSOR / p55 / chromatin / epigenetics / WD4 / histone / Chromatin regulator / Nucleus / Phosphoprotein / Repressor / Transcription / Transcription regulation / WD repeat / Chromosomal protein / DNA-binding / Nucleosome core / NUCLEAR PROTEIN | ||||||
Function / homology | Function and homology information G0 and Early G1 / HDACs deacetylate histones / Regulation of TP53 Activity through Acetylation / Oxidative Stress Induced Senescence / Transcriptional Regulation by E2F6 / Regulation of PTEN gene transcription / Neddylation / RCAF complex / eggshell chorion gene amplification / Myb complex ...G0 and Early G1 / HDACs deacetylate histones / Regulation of TP53 Activity through Acetylation / Oxidative Stress Induced Senescence / Transcriptional Regulation by E2F6 / Regulation of PTEN gene transcription / Neddylation / RCAF complex / eggshell chorion gene amplification / Myb complex / segment specification / CAF-1 complex / polytene chromosome / facultative heterochromatin formation / nucleosome organization / NURF complex / NuRD complex / DNA replication-dependent chromatin assembly / ESC/E(Z) complex / histone methyltransferase complex / nuclear chromosome / Sin3-type complex / regulation of mitotic cell cycle / heterochromatin formation / nucleosome assembly / structural constituent of chromatin / histone deacetylase binding / nucleosome / chromosome / chromatin organization / histone binding / transcription regulator complex / nucleic acid binding / transcription cis-regulatory region binding / chromatin remodeling / protein heterodimerization activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | ||||||
Authors | Song, J.J. / Garlick, J.D. / Kingston, R.E. | ||||||
Citation | Journal: Genes Dev. / Year: 2008 Title: Structural basis of histone H4 recognition by p55. Authors: Song, J.J. / Garlick, J.D. / Kingston, R.E. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3c9c.cif.gz | 85.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3c9c.ent.gz | 64.8 KB | Display | PDB format |
PDBx/mmJSON format | 3c9c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c9/3c9c ftp://data.pdbj.org/pub/pdb/validation_reports/c9/3c9c | HTTPS FTP |
---|
-Related structure data
Related structure data | 3c99SC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 48817.715 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Caf1 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf9 / References: UniProt: Q24572 |
---|---|
#2: Protein/peptide | Mass: 3191.853 Da / Num. of mol.: 1 / Fragment: UNP residues 16-42 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence is found naturally in Drosophila melanogaster. References: UniProt: P84040 |
#3: Chemical | ChemComp-CD / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 6.219 Å3/Da / Density % sol: 80.2 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 110 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12B / Wavelength: 0.9 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 6, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. obs: 21402 / % possible obs: 99.7 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.11 / Rsym value: 0.11 / Net I/σ(I): 28.4 |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 6 % / Rmerge(I) obs: 0.591 / Mean I/σ(I) obs: 3.9 / Rsym value: 0.591 / % possible all: 100 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3c99 Resolution: 3.2→50 Å
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→50 Å
| ||||||||||||||||||||
Refine LS restraints |
|