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Open data
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Basic information
Entry | Database: PDB / ID: 2zh2 | ||||||
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Title | Complex structure of AFCCA with tRNAminiDAC | ||||||
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![]() | TRANSFERASE/RNA / ATP-binding / Magnesium / Metal-binding / Nucleotide-binding / Nucleotidyltransferase / RNA repair / RNA-binding / tRNA processing / TRANSFERASE-RNA complex | ||||||
Function / homology | ![]() tRNA surveillance / CCACCA tRNA nucleotidyltransferase activity / CCA tRNA nucleotidyltransferase / tRNA 3'-terminal CCA addition / RNA repair / tRNA binding / magnesium ion binding / ATP binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Toh, Y. / Tomita, K. | ||||||
![]() | ![]() Title: Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme Authors: Toh, Y. / Numata, T. / Watanabe, K. / Takeshita, D. / Nureki, O. / Tomita, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.5 KB | Display | ![]() |
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PDB format | ![]() | 93.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463.7 KB | Display | ![]() |
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Full document | ![]() | 476.8 KB | Display | |
Data in XML | ![]() | 20.9 KB | Display | |
Data in CIF | ![]() | 29.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2zh1C ![]() 2zh3C ![]() 2zh4C ![]() 2zh5C ![]() 2zh6C ![]() 2zh7C ![]() 2zh8C ![]() 2zh9C ![]() 2zhaC ![]() 2zhbC ![]() 2drbS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 10928.526 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
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#2: Protein | Mass: 51469.137 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.56 % | ||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7.5 Details: 50mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM NH4SO4, pH 7.5, VAPOR DIFFUSION, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 27, 2006 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.66→40 Å / Num. obs: 23187 / % possible obs: 100 % / Redundancy: 13.6 % / Biso Wilson estimate: 43.6 Å2 / Rsym value: 0.15 / Net I/σ(I): 50.5 |
Reflection shell | Resolution: 2.66→2.76 Å / Redundancy: 12.8 % / Mean I/σ(I) obs: 11.3 / Num. unique all: 2258 / Rsym value: 0.268 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2DRB Resolution: 2.66→39.94 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 2763048.31 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 29.6244 Å2 / ksol: 0.34772 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.66→39.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.66→2.83 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 6
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Xplor file |
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