SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
L-TYROSINE (TYR): 3 TYROSINE LIGANDS ARE BOUND IN THE TWO MONOMERS OF THE ASYMMETRIC UNIT, 1 IN ...L-TYROSINE (TYR): 3 TYROSINE LIGANDS ARE BOUND IN THE TWO MONOMERS OF THE ASYMMETRIC UNIT, 1 IN SUBUNIT A AND 2 IN SUBUNIT B.
-
実験情報
-
実験
実験
手法: X線回折 / 使用した結晶の数: 1
-
試料調製
結晶
マシュー密度: 3.92 Å3/Da / 溶媒含有率: 68.6 % / 解説: NONE
結晶化
pH: 7.5 詳細: 0.1 M TRIS-HCL PH 7.5, 1.5 M AMMONIUM SULFATE, 12% V/V GLYCEROL
解像度: 2.3→44.02 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.939 / SU B: 6.144 / SU ML: 0.078 / 交差検証法: THROUGHOUT / ESU R: 0.032 / ESU R Free: 0.028 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. CHAIN A RESIDUES 11-12 AND CHAIN B RESIDUES 10-12 ARE DISORDERED.
Rfactor
反射数
%反射
Selection details
Rfree
0.16375
3380
5 %
RANDOM
Rwork
0.13923
-
-
-
obs
0.14046
64572
96.94 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK