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- PDB-2yn7: Crystal structure of an outer surface protein BBA66 from Borrelia... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2yn7 | ||||||
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Title | Crystal structure of an outer surface protein BBA66 from Borrelia burgdorferi | ||||||
![]() | OUTER SURFACE PROTEIN | ||||||
![]() | MEMBRANE PROTEIN / OUTER SURFACE LIPOPROTEIN | ||||||
Function / homology | Bbcrasp-1 / Bbcrasp-1 / Borrelia lipoprotein paralogus family 54/60 / Borrelia Bbcrasp-1 domain containing protein / Orthogonal Bundle / Mainly Alpha / membrane / Outer surface protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brangulis, K. / Petrovskis, I. / Kazaks, A. / Ranka, R. / Baumanis, V. / Tars, K. | ||||||
![]() | ![]() Title: Crystal Structure of the Infectious Phenotype-Associated Outer Surface Protein Bba66 from the Lyme Disease Agent Borrelia Burgdorferi. Authors: Brangulis, K. / Petrovskis, I. / Kazaks, A. / Tars, K. / Ranka, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97.4 KB | Display | ![]() |
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PDB format | ![]() | 75.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435 KB | Display | ![]() |
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Full document | ![]() | 442.3 KB | Display | |
Data in XML | ![]() | 17.4 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4axzS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25989.285 Da / Num. of mol.: 2 / Fragment: RESIDUES 191-411 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47 % / Description: NONE |
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Crystal grow | pH: 9 / Details: 18% PEG 2000 MME, 0.1 M TRIS PH 9.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jul 1, 2012 / Details: RH-COATED TOROIDAL SI MIRROR |
Radiation | Monochromator: DOUBLE CRYSTAL SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.24→33.01 Å / Num. obs: 21363 / % possible obs: 94.3 % / Observed criterion σ(I): 2.4 / Redundancy: 1.9 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 2.24→2.36 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.29 / Mean I/σ(I) obs: 2.2 / % possible all: 85.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4AXZ Resolution: 2.25→29.78 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.921 / SU B: 6.255 / SU ML: 0.154 / Cross valid method: THROUGHOUT / ESU R: 0.335 / ESU R Free: 0.226 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT. U VALUES REFINED INDIVIDUALLY.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.029 Å2
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Refinement step | Cycle: LAST / Resolution: 2.25→29.78 Å
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Refine LS restraints |
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