SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 10-STRANDED BARREL THIS IS REPRESENTED BY A 11-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
ETHYLENE GLYCOL (EDO): ETHYLENE GLYCOL WAS USED AS A CRYOPROTECTANT (2R)-2-HYDROXY-3-(4- ...ETHYLENE GLYCOL (EDO): ETHYLENE GLYCOL WAS USED AS A CRYOPROTECTANT (2R)-2-HYDROXY-3-(4-HYDROXYPHENYL)PROPANOIC ACID (34H): 4-HYDROXYPHENYLPYRUVATE WAS COVALENTLY LINKED THROUGH ITS C2 ATOM TO THE SG ATOM OF CYS215 TO GIVE A THIOHEMIKETAL SPECIES FORMIC ACID (FMT): SODIUM FORMATE WAS A COMPONENT OF THE PRECIPITANT
配列の詳細
THERE IS AN ADDITIONAL TRIPEPTIDE (WITH SEQUENCE GLY-SER- HIS) AT THE N-TERMINUS LEFT OVER AFTER ...THERE IS AN ADDITIONAL TRIPEPTIDE (WITH SEQUENCE GLY-SER- HIS) AT THE N-TERMINUS LEFT OVER AFTER PROTEOLYTIC CLEAVAGE OF THE HIS-TAG
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.12 Å3/Da / 溶媒含有率: 60.6 % / 解説: NONE
結晶化
手法: 蒸気拡散法 / pH: 6.5 詳細: VAPOR DIFFUSION. PROTEIN (10 MG/ML) MIXED WITH AN EQUAL VOLUME OF 3 M SODIUM FORMATE, 2 MM DITHIOTHREITOL,100 MM HEPES PH (6.5).
解像度: 2.22→43.85 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.941 / SU B: 4.237 / SU ML: 0.106 / 交差検証法: THROUGHOUT / ESU R: 0.192 / ESU R Free: 0.165 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.20959
1169
5.1 %
RANDOM
Rwork
0.17386
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obs
0.17572
21654
99.1 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK