TRANSPORT PROTEIN / CELL MEMBRANE / ION TRANSPORT / PORIN
Function / homology
Function and homology information
porin activity / pore complex / monoatomic ion transmembrane transport / cell outer membrane Similarity search - Function
Porin, gammaproteobacterial / Porin, Gram-negative type, conserved site / General diffusion Gram-negative porins signature. / Porin domain, Gram-negative type / Gram-negative porin / Porin, Gram-negative type / Porin / Porin domain superfamily / Porin / Beta Barrel / Mainly Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 30 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THIS IS FROM A CLINICAL SEQUENCE DESCRIBED IN PUBMED ID: 11722730. A.S.LOW,F.M.MACKENZIE,I.M.GOULD, ...THIS IS FROM A CLINICAL SEQUENCE DESCRIBED IN PUBMED ID: 11722730. A.S.LOW,F.M.MACKENZIE,I.M.GOULD, I.R.BOOTH. PROTECTED ENVIRONMENTS ALLOW PARALLEL EVOLUTION OF A BACTERIAL PATHOGEN IN A PATIENT SUBJECTED TO LONG-TERM ANTIBIOTIC THERAPY. MOL.MICROBIOL. 42,619
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.77 Å3/Da / Density % sol: 67.37 % / Description: NONE
Resolution: 2.85→49.88 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.918 / SU B: 28.482 / SU ML: 0.254 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 1.005 / ESU R Free: 0.328 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.234
1301
5.1 %
RANDOM
Rwork
0.214
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obs
0.215
24149
96.4 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK