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- PDB-2w0n: Plasticity of PAS domain and potential role for signal transducti... -

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Basic information

Entry
Database: PDB / ID: 2w0n
TitlePlasticity of PAS domain and potential role for signal transduction in the histidine-kinase DcuS
ComponentsSENSOR PROTEIN DCUS
KeywordsTRANSFERASE / SIGNAL TRANSDUCTION / TWO-COMPONENT REGULATORY SYSTEM / PAS / DCUS / KINASE / MEMBRANE / SOLID STATE NMR / CELL INNER MEMBRANE / CELL MEMBRANE / TRANSMEMBRANE / PHOSPHOPROTEIN
Function / homology
Function and homology information


protein histidine kinase complex / protein histidine kinase activity / histidine kinase / phosphorelay signal transduction system / phosphorelay sensor kinase activity / outer membrane-bounded periplasmic space / regulation of DNA-templated transcription / signal transduction / ATP binding / identical protein binding ...protein histidine kinase complex / protein histidine kinase activity / histidine kinase / phosphorelay signal transduction system / phosphorelay sensor kinase activity / outer membrane-bounded periplasmic space / regulation of DNA-templated transcription / signal transduction / ATP binding / identical protein binding / plasma membrane / cytosol
Similarity search - Function
SpoOB, alpha-helical domain / Sensor_kinase_SpoOB-type, alpha-helical domain / Single cache domain 3 / Single cache domain 3 / Signal transduction histidine kinase, sporulation regulator SpoOB / Periplasmic sensor-like domain superfamily / PAS domain / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. ...SpoOB, alpha-helical domain / Sensor_kinase_SpoOB-type, alpha-helical domain / Single cache domain 3 / Single cache domain 3 / Signal transduction histidine kinase, sporulation regulator SpoOB / Periplasmic sensor-like domain superfamily / PAS domain / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / Beta-Lactamase / PAS fold / PAS fold / PAS domain / PAS repeat profile. / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / PAS domain / Histidine kinase-like ATPases / PAS domain superfamily / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Sensor histidine kinase DcuS
Similarity search - Component
Biological speciesESCHERICHIA COLI (E. coli)
MethodSOLID-STATE NMR
AuthorsEtzkorn, M. / Kneuper, H. / Duennwald, P. / Vijayan, V. / Kraemer, J. / Griesinger, C. / Becker, S. / Unden, G. / Baldus, M.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2008
Title: Plasticity of the Pas Domain and a Potential Role for Signal Transduction in the Histidine Kinase Dcus.
Authors: Etzkorn, M. / Kneuper, H. / Duennwald, P. / Vijayan, V. / Kraemer, J. / Griesinger, C. / Becker, S. / Unden, G. / Baldus, M.
History
DepositionAug 19, 2008Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 30, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 24, 2013Group: Database references / Derived calculations ...Database references / Derived calculations / Experimental preparation / Other / Source and taxonomy / Version format compliance
Revision 1.2May 15, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_mr

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MolmilJmol/JSmol

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Assembly

Deposited unit
A: SENSOR PROTEIN DCUS


Theoretical massNumber of molelcules
Total (without water)13,2841
Polymers13,2841
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / -
Representative

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Components

#1: Protein SENSOR PROTEIN DCUS


Mass: 13283.904 Da / Num. of mol.: 1 / Fragment: DCUS-PASC, RESIDUES 211-325
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) ESCHERICHIA COLI (E. coli) / Plasmid: PMW309 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P0AEC8, histidine kinase
Sequence detailsTHE THREE N TERMINAL RESIDUES G,H,M ARE INSERTED AND NOT PART OF THE NATIVE SEQUENCE.

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Experimental details

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Experiment

ExperimentMethod: SOLID-STATE NMR

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Processing

NMR ensembleConformers submitted total number: 10

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