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Open data
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Basic information
Entry | Database: PDB / ID: 2sak | ||||||
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Title | STAPHYLOKINASE (SAKSTAR VARIANT) | ||||||
![]() | STAPHYLOKINASE | ||||||
![]() | PLASMINOGEN ACTIVATOR / PLASMINOGEN ACTIVATION / FIBRINOLYSIS / STAPHYLOKINASE / HYDROLASE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rabijns, A. / De Bondt, H.L. / De Maeyer, M. / Lasters, I. / De Ranter, C. | ||||||
![]() | ![]() Title: Three-dimensional structure of staphylokinase, a plasminogen activator with therapeutic potential. Authors: Rabijns, A. / De Bondt, H.L. / De Ranter, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38 KB | Display | ![]() |
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PDB format | ![]() | 25.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13898.865 Da / Num. of mol.: 1 / Mutation: SAKSTAR VARIANT / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-TRS / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 0.2M MGCL2, O.1M TRIS BUFFER PH 8.5 AND 30 % PEG 4K, USING MICROSEEDING TECHNIQUES. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging dropDetails: Rabijins, A., (1997) Protein.Struct.Funct.Genet., 27, 160. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 1, 1995 / Details: MIRRORS |
Radiation | Monochromator: GE(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→15 Å / Num. obs: 11949 / % possible obs: 95.8 % / Observed criterion σ(I): 2 / Redundancy: 6.4 % / Rsym value: 0.034 / Net I/σ(I): 25 |
Reflection shell | Resolution: 1.8→1.82 Å / Redundancy: 4.3 % / Mean I/σ(I) obs: 8.5 / Rsym value: 0.119 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 76483 / Rmerge(I) obs: 0.034 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 19.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.88 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.3 |