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- PDB-2rmf: Human Urocortin 1 -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 2rmf
TitleHuman Urocortin 1
ComponentsUrocortin
KeywordsHORMONE / CRF ligand / Sauvagine / Astressin2B / Urocortins / Urotensins / CRF receptors / Amidation / Cleavage on pair of basic residues / Secreted / NEUROPEPTIDE
Function / homology
Function and homology information


histone deacetylase inhibitor activity / corticotropin-releasing hormone receptor 2 binding / positive regulation of corticotropin secretion / positive regulation of behavioral fear response / negative regulation of hormone secretion / varicosity / response to auditory stimulus / drinking behavior / positive regulation of cAMP-mediated signaling / negative regulation of appetite ...histone deacetylase inhibitor activity / corticotropin-releasing hormone receptor 2 binding / positive regulation of corticotropin secretion / positive regulation of behavioral fear response / negative regulation of hormone secretion / varicosity / response to auditory stimulus / drinking behavior / positive regulation of cAMP-mediated signaling / negative regulation of appetite / positive regulation of vascular permeability / neuropeptide hormone activity / negative regulation of cell size / Class B/2 (Secretin family receptors) / response to pain / negative regulation of feeding behavior / positive regulation of calcium ion import / startle response / associative learning / social behavior / corticotropin-releasing hormone receptor 1 binding / positive regulation of collagen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / neuropeptide signaling pathway / axon terminus / response to glucocorticoid / regulation of synaptic transmission, glutamatergic / positive regulation of cardiac muscle contraction / negative regulation of blood pressure / aerobic respiration / positive regulation of DNA replication / positive regulation of translation / female pregnancy / sensory perception of sound / vasodilation / positive regulation of interleukin-6 production / neuron projection development / positive regulation of peptidyl-serine phosphorylation / response to estradiol / positive regulation of cell growth / perikaryon / response to oxidative stress / negative regulation of neuron apoptotic process / G protein-coupled receptor signaling pathway / negative regulation of gene expression / dendrite / positive regulation of transcription by RNA polymerase II / extracellular region
Similarity search - Function
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1920 / Urocortin/corticotropin-releasing factor / Corticotropin-releasing factor conserved site / Corticotropin-releasing factor family signature. / corticotropin-releasing factor / Corticotropin-releasing factor / Corticotropin-releasing factor family / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Helix non-globular / Special
Similarity search - Domain/homology
MethodSOLUTION NMR / torsion angle dynamics
AuthorsGrace, C.R.R. / Perrin, M.H. / Cantle, J.P. / Vale, W.W. / Rivier, J.E. / Riek, R.
CitationJournal: J.Am.Chem.Soc. / Year: 2007
Title: Common and divergent structural features of a series of corticotropin releasing factor-related peptides
Authors: Grace, C.R.R. / Perrin, M.H. / Cantle, J.P. / Vale, W.W. / Rivier, J.E. / Riek, R.
History
DepositionOct 16, 2007Deposition site: BMRB / Processing site: PDBJ
Revision 1.0Jan 1, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Mar 16, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Urocortin


Theoretical massNumber of molelcules
Total (without water)4,7031
Polymers4,7031
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100target function
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide Urocortin / HUcn1


Mass: 4703.277 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Solid-phase approach / References: UniProt: P55089

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H TOCSY
1212D DQF-COSY
1312D 1H-1H NOESY

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Sample preparation

DetailsContents: 1.5mM Human Ucn1, DMSO / Solvent system: DMSO
SampleConc.: 1.5 mM / Component: Human Ucn1
Sample conditionsPressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometerType: Bruker INOVA / Manufacturer: Bruker / Model: INOVA / Field strength: 700 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CYANA1.0.6Guntert, Mumenthaler and Wuthrichstructure solution
CYANA1.0.6Guntert, Mumenthaler and Wuthrichrefinement
RefinementMethod: torsion angle dynamics / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20

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