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- PDB-2ra6: Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin a... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ra6 | ||||||
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Title | Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6 with Bound 4-ethylphenol | ||||||
![]() | Trichosurin | ||||||
![]() | TRANSPORT PROTEIN / Lipocalin / beta Barrel / Glycoprotein / Milk protein / Secreted / Transport | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Watson, R.P. | ||||||
![]() | ![]() Title: Three-dimensional structure and ligand binding properties of trichosurin, a metatherian lipocalin from the milk whey of the common brushtail possum Trichosurus vulpecula Authors: Watson, R.P. / Demmer, J. / Baker, E.N. / Arcus, V.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 138.9 KB | Display | ![]() |
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PDB format | ![]() | 108.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2r73C ![]() 2r74SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 19709.904 Da / Num. of mol.: 4 / Mutation: G102E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: pET23a / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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-Non-polymers , 5 types, 407 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/ETY.gif)
![](data/chem/img/IPA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/ETY.gif)
![](data/chem/img/IPA.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-CL / #4: Chemical | ChemComp-ETY / #5: Chemical | ChemComp-IPA / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.58 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 100mM Sodium Acetate, 200mM ZnCl2, 8% 2-propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Sep 1, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.89 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. all: 118308 / Num. obs: 117706 / % possible obs: 99.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.072 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 1.5→1.6 Å / Rmerge(I) obs: 0.289 / Mean I/σ(I) obs: 4.3 / % possible all: 96 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2R74 Resolution: 1.5→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.5→20 Å
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Refine LS restraints |
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