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- PDB-2r2g: Structure of Eugenol Synthase from Ocimum basilicum complexed wit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2r2g | ||||||
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Title | Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF | ||||||
![]() | Eugenol synthase 1 | ||||||
![]() | PLANT PROTEIN / eugenol / phenylpropene / PIP reductase / short-chain dehydrogenase/reductase | ||||||
Function / homology | ![]() eugenol synthase / eugenol biosynthetic process / oxidoreductase activity / nucleotide binding / protein homodimerization activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Louie, G.V. / Noel, J.P. / Bowman, M.E. | ||||||
![]() | ![]() Title: Structure and reaction mechanism of basil eugenol synthase Authors: Louie, G.V. / Baiga, T.J. / Bowman, M.E. / Koeduka, T. / Taylor, J.H. / Spassova, S.M. / Pichersky, E. / Noel, J.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 146.1 KB | Display | ![]() |
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PDB format | ![]() | 113.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1009.2 KB | Display | ![]() |
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Full document | ![]() | 1021.8 KB | Display | |
Data in XML | ![]() | 29.4 KB | Display | |
Data in CIF | ![]() | 41 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2qw8C ![]() 2qx7C ![]() 2qysC ![]() 2qzzC ![]() 2r6jC ![]() 1qycS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 |
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Unit cell |
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Details | biological unit is a monomer (half of the asymmetric unit) |
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Components
#1: Protein | Mass: 35988.414 Da / Num. of mol.: 2 / Mutation: K132Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.95 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.1 M sodium succinate, 21% PEG 3350, 0.3 M KCl, 2 mM dithiothreitol, 5 mM NADP+, pH 5.5, vapor diffusion, hanging drop, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 12, 2006 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.8→44.151 Å / Num. obs: 57391 / % possible obs: 92.1 % / Redundancy: 2.9 % / Rmerge(I) obs: 0.109 / Rsym value: 0.109 / Net I/σ(I): 4.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QYC Resolution: 1.8→44.09 Å / FOM work R set: 0.816 / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 47.148 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.304 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→44.09 Å
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Refine LS restraints |
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Xplor file |
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