ImmunoglobulinG-bindingproteinG / IgG-binding protein G
Mass: 5941.474 Da / Num. of mol.: 1 / Fragment: First Immunoglobin binding domain (GB1) Source method: isolated from a genetically manipulated source Details: deposited only main chain atoms / Source: (gene. exp.) Streptococcus sp. 'group G' (bacteria) / Strain: group G / Gene: spg / Production host: Escherichia coli (E. coli) / References: UniProt: P19909, UniProt: P06654*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
HCN-HSQC
2
2
2
water flip-back 3D 15N-edited 1H-1H COSYHMQC
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Sample preparation
Details
Contents: perdeuterated (triple labelled 15N, 13C, 2D) Solvent system: Partially aligned in bacteriophage and lyotrophic alcohol medium.
Sample conditions
Ionic strength: 50mM salt / pH: 5.6 / Pressure: ambient / Temperature: 300 K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz
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Processing
NMR software
Name
Version
Developer
Classification
Dynamic Meccano
1
bouvigniesetal
structuresolution
Dynamic Meccano
1
bouvigniesetal
refinement
Refinement
Method: dynamic meccano / Software ordinal: 1 Details: Ultrahigh-resolution backbone structure of perdeuterated protein GB1 using residual dipolar couplings from two alignment media. Angew Chem Int Ed Engl. 2006 Dec 11;45(48):8166-9.
NMR ensemble
Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 1 / Conformers submitted total number: 1
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