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- PDB-2o32: Solution structure of U2 snRNA stem I from human, containing modi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2o32 | ||||||||||||||||||
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Title | Solution structure of U2 snRNA stem I from human, containing modified nucleotides | ||||||||||||||||||
![]() | 5'-R(*![]() RNA / Wobble base-pairs / Tetraloop | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / simulated annealing, molecular dynamics torsion angle dynamics | ![]() Sashital, D.G. / Venditti, V. / Angers, C.G. / Cornilescu, G. / Butcher, S.E. | ![]() ![]() Title: Structure and thermodynamics of a conserved U2 snRNA domain from yeast and human. Authors: Sashital, D.G. / Venditti, V. / Angers, C.G. / Cornilescu, G. / Butcher, S.E. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 252.8 KB | Display | ![]() |
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PDB format | ![]() | 211.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 326.6 KB | Display | ![]() |
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Full document | ![]() | 455.2 KB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 33.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 6393.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Human U2 snRNA stem I |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D NOESY, 2D TOCSY, and natural 13C abundance 2D HSQC. |
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Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 750 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics torsion angle dynamics Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |