ソフトウェア 名称 バージョン 分類 NB XSCALEデータスケーリング Show MOLREP位相決定 Show RESOLVE2.08 位相決定 Show REFMAC精密化 Show PDB_EXTRACT2 データ抽出 Show ProDCデータ収集 XDSデータ削減
精密化 構造決定の手法 : 分子置換開始モデル : PDB ENTRY 1w3n解像度 : 1.8→29.35 Å / Cor.coef. Fo :Fc : 0.974 / Cor.coef. Fo :Fc free : 0.966 / SU B : 4.249 / SU ML : 0.064 / TLS residual ADP flag : LIKELY RESIDUALIsotropic thermal model : isotropic B factor refinement in combination with TLS refinement交差検証法 : THROUGHOUT / σ(F) : 0 / ESU R : 0.071 / ESU R Free : 0.073 / 立体化学のターゲット値 : MAXIMUM LIKELIHOOD詳細 : HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS, Reported Average Isotropic B Value is without TLS contributionRfactor 反射数 %反射 Selection details Rfree 0.179 5421 5 % RANDOM Rwork 0.157 - - - obs 0.158 107995 99.96 % - all - 108033 - -
溶媒の処理 イオンプローブ半径 : 0.8 Å / 減衰半径 : 0.8 Å / VDWプローブ半径 : 1.2 Å / 溶媒モデル : BABINET MODEL WITH MASK原子変位パラメータ Biso mean : 13.196 Å2 Baniso -1 Baniso -2 Baniso -3 1- 2.18 Å2 1.09 Å2 0 Å2 2- - 2.18 Å2 0 Å2 3- - - -3.27 Å2
精密化ステップ サイクル : LAST / 解像度 : 1.8→29.35 Åタンパク質 核酸 リガンド 溶媒 全体 原子数 4598 0 1 410 5009
拘束条件 大きな表を表示 (5 x 25) 大きな表を隠す Refine-ID タイプ Dev ideal Dev ideal target 数 X-RAY DIFFRACTION r_bond_refined_d0.012 0.022 4736 X-RAY DIFFRACTION r_bond_other_d0.001 0.02 3238 X-RAY DIFFRACTION r_angle_refined_deg1.472 1.995 6429 X-RAY DIFFRACTION r_angle_other_deg0.891 3 7970 X-RAY DIFFRACTION r_dihedral_angle_1_deg3.898 5 578 X-RAY DIFFRACTION r_dihedral_angle_2_deg34.432 24.808 208 X-RAY DIFFRACTION r_dihedral_angle_3_deg11.35 15 855 X-RAY DIFFRACTION r_dihedral_angle_4_deg12.683 15 20 X-RAY DIFFRACTION r_chiral_restr0.101 0.2 733 X-RAY DIFFRACTION r_gen_planes_refined0.005 0.02 5168 X-RAY DIFFRACTION r_gen_planes_other0.001 0.02 892 X-RAY DIFFRACTION r_nbd_refined0.211 0.3 986 X-RAY DIFFRACTION r_nbd_other0.186 0.3 3479 X-RAY DIFFRACTION r_nbtor_refined0.185 0.5 2370 X-RAY DIFFRACTION r_nbtor_other0.09 0.5 2362 X-RAY DIFFRACTION r_xyhbond_nbd_refined0.144 0.15 537 X-RAY DIFFRACTION r_metal_ion_refined0.025 0.5 1 X-RAY DIFFRACTION r_symmetry_vdw_refined0.103 0.3 10 X-RAY DIFFRACTION r_symmetry_vdw_other0.174 0.3 17 X-RAY DIFFRACTION r_symmetry_hbond_refined0.143 0.15 29 X-RAY DIFFRACTION r_mcbond_it4.23 1.5 3764 X-RAY DIFFRACTION r_mcbond_other1.28 1.5 1152 X-RAY DIFFRACTION r_mcangle_it4.454 2 4721 X-RAY DIFFRACTION r_scbond_it8.597 3 2143 X-RAY DIFFRACTION r_scangle_it11.714 4.5 1708
LS精密化 シェル 解像度 : 1.8→1.847 Å / Total num. of bins used : 20 Rfactor 反射数 %反射 Rfree 0.317 407 - Rwork 0.27 7456 - obs - 7863 99.81 %
精密化 TLS 手法 : refined / Refine-ID : X-RAY DIFFRACTION
大きな表を表示 (25 x 20) 大きな表を隠す ID L11 (°2 )L12 (°2 )L13 (°2 )L22 (°2 )L23 (°2 )L33 (°2 )S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2 )T12 (Å2 )T13 (Å2 )T22 (Å2 )T23 (Å2 )T33 (Å2 )Origin x (Å)Origin y (Å)Origin z (Å)1 4.2846 4.5921 -2.4064 10.2562 -5.2474 7.1433 -0.0448 0.0874 -0.2054 -0.2913 -0.0357 0.049 0.171 -0.3494 0.0804 0.1669 0.0628 -0.024 0.1742 -0.0506 0.2092 -6.5593 70.8748 37.9188 2 1.3757 0.1409 0.2256 2.2029 0.3503 3.5837 0.0713 0.0329 -0.1477 -0.2789 -0.0987 0.1571 0.2231 -0.0292 0.0274 0.1898 0.0633 -0.0063 0.1444 -0.03 0.2103 -3.3613 68.4507 36.084 3 0.4772 0.1471 0.2921 1.4536 0.6596 3.1916 0.0684 0.027 -0.0852 -0.1252 -0.0388 -0.1488 0.2134 0.1984 -0.0296 0.1535 0.0783 0.0219 0.1635 -0.0074 0.2018 7.4561 67.4708 41.3436 4 9.8185 -3.3785 -0.6826 14.6926 6.6272 11.6587 0.0681 -0.2275 0.2961 0.1478 0.071 -0.6636 -0.481 0.5605 -0.1391 0.1326 -0.0138 -0.0517 0.2364 0.0244 0.2667 16.4144 79.2245 56.0082 5 2.8657 -1.2604 -1.098 2.6857 0.7768 4.0674 0.0937 -0.0055 0.1161 -0.1421 -0.0268 -0.4537 -0.1412 0.5412 -0.0669 0.0818 0.0226 0.0272 0.174 0.0123 0.196 14.1885 77.6191 43.9739 6 4.6112 -2.9363 -0.6846 6.0559 2.2112 4.3909 -0.039 0.0388 0.2617 -0.4689 0.037 -0.7236 -0.4179 0.6196 0.002 0.1479 0.0192 0.0404 0.0819 0.0327 0.1633 10.9233 87.8966 37.8037 7 1.5583 1.3911 0.1248 3.0144 -0.1646 1.6865 -0.0763 0.1511 -0.0243 -0.6542 -0.0235 0.0871 -0.2381 -0.0393 0.0998 0.2663 0.0975 -0.0287 0.1002 -0.0142 0.1162 -2.4581 86.41 30.4938 8 5.8918 3.2941 -5.2107 4.8122 -4.3528 14.0368 -0.2705 0.4579 0.1744 -0.4684 0.1348 0.2872 -0.6578 -0.6917 0.1358 0.3464 0.1165 -0.1426 0.1423 -0.0226 0.2426 -9.4978 92.7344 29.9249 9 2.0514 -0.0969 -0.1181 3.6185 -0.3039 4.3778 0.014 -0.0103 0.0482 -0.1388 -0.0278 0.4354 -0.0412 -0.6984 0.0139 0.0855 0.0279 -0.0475 0.2454 -0.0441 0.2545 -16.874 75.8216 41.7965 10 15.7196 4.8537 -18.0536 5.7129 -6.9609 34.9821 -0.5618 0.9103 -0.1112 -0.8265 0.2813 0.6742 0.1112 -1.6497 0.2805 0.2787 0.1705 -0.2333 0.438 0.0072 0.4626 -20.4773 87.2189 29.5686 11 1.9455 -0.6552 -0.6166 2.0397 0.8505 4.8347 0.0699 -0.0212 -0.2648 0.2314 -0.1148 -0.1953 0.4746 0.4002 0.0449 0.1387 0.003 -0.0298 0.1091 0.0385 0.2001 10.2176 63.6286 71.9535 12 1.0445 0.1668 -0.3504 2.0622 1.3828 4.9163 0.093 0.0009 -0.2127 0.1681 -0.0892 -0.0873 0.4523 0.0305 -0.0038 0.1286 -0.0147 -0.0045 0.1032 0.0266 0.1725 3.8114 65.3847 68.4908 13 0.8971 0.135 0.0118 0.987 -0.6077 3.2837 0.068 -0.0127 -0.1351 0.0878 -0.1029 0.0897 0.3296 -0.2085 0.0349 0.1518 -0.0525 0.0123 0.1264 -0.0029 0.1864 -4.4801 64.2881 65.4064 14 22.7864 3.148 -1.8681 15.6851 -3.8711 18.3903 -0.078 0.6114 0.5133 -0.3181 0.0589 0.338 -0.4546 -0.672 0.0191 0.1767 0.0586 -0.0495 0.2413 -0.0299 0.2518 -12.6036 81.1324 53.0074 15 1.7131 0.8634 -0.0964 2.6211 -0.4393 3.0971 0.0759 0.057 0.0291 0.0716 -0.0868 0.3741 -0.0172 -0.4708 0.0109 0.0756 -0.0151 0.0153 0.1662 -0.0216 0.1864 -10.3801 74.434 64.3126 16 1.43 1.3137 0.2435 4.2563 0.8385 2.5467 0.0763 -0.0411 0.223 0.3118 -0.235 0.596 -0.2631 -0.5105 0.1587 0.1255 0.0013 0.0432 0.126 -0.0364 0.1859 -9.1004 82.2914 72.2529 17 1.4455 -0.4257 -0.1987 1.8173 0.1738 2.4475 0.0014 -0.1648 -0.1016 0.4778 -0.0474 -0.0118 -0.0679 0.0506 0.046 0.1992 -0.0773 -0.0033 0.0826 0.0266 0.1114 3.7328 79.3476 80.427 18 5.0149 -3.1028 -5.8117 4.098 1.9978 16.659 -0.0711 -0.5792 -0.0969 0.6288 0.1129 -0.2923 -0.9085 0.9187 -0.0417 0.3142 -0.103 -0.1034 0.2091 0.0122 0.1985 12.5739 85.2271 83.4163 19 2.3991 0.4474 -0.1462 3.1138 0.0947 5.2702 -0.0034 -0.1291 -0.0372 0.0399 -0.0805 -0.423 0.0367 0.7934 0.084 0.0974 0.0398 -0.0339 0.2973 0.0435 0.2786 19.7984 71.883 67.0563 20 4.1801 -1.5841 -5.4346 3.292 -1.1204 10.8222 0.1619 -0.6229 0.0317 0.4932 -0.0667 -0.2402 0.0414 1.2135 -0.0953 0.4935 -0.1147 -0.0792 0.7377 -0.0014 0.5616 24.0424 79.6942 81.3554
精密化 TLSグループ Refine-ID : X-RAY DIFFRACTION / Selection : ALL
大きな表を表示 (6 x 20) 大きな表を隠す ID Refine TLS-ID Auth asym-ID Label asym-ID Auth seq-ID Label seq-ID 1 1 AA1 - 16 1 - 16 2 2 AA17 - 56 17 - 56 3 3 AA57 - 104 57 - 104 4 4 AA105 - 113 105 - 113 5 5 AA114 - 149 114 - 149 6 6 AA150 - 173 150 - 173 7 7 AA174 - 211 174 - 211 8 8 AA212 - 233 212 - 233 9 9 AA234 - 273 234 - 273 10 10 AA274 - 288 274 - 288 11 11 BB1 - 30 1 - 30 12 12 BB31 - 56 31 - 56 13 13 BB57 - 104 57 - 104 14 14 BB105 - 110 105 - 110 15 15 BB111 - 142 111 - 142 16 16 BB143 - 168 143 - 168 17 17 BB169 - 210 169 - 210 18 18 BB211 - 233 211 - 233 19 19 BB234 - 272 234 - 272 20 20 BB273 - 288 273 - 288