- PDB-2n7i: NMR structure of the prolactin receptor transmembrane domain -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 2n7i
タイトル
NMR structure of the prolactin receptor transmembrane domain
要素
Prolactin receptor
キーワード
HORMONE RECEPTOR / transmembrane domain / membrane protein / receptor
機能・相同性
機能・相同性情報
prolactin receptor activity / regulation of epithelial cell differentiation / prostate gland growth / mammary gland epithelial cell differentiation / steroid biosynthetic process / activation of Janus kinase activity / cellular response to granulocyte macrophage colony-stimulating factor stimulus / activation of transmembrane receptor protein tyrosine kinase activity / Prolactin receptor signaling / cytokine binding ...prolactin receptor activity / regulation of epithelial cell differentiation / prostate gland growth / mammary gland epithelial cell differentiation / steroid biosynthetic process / activation of Janus kinase activity / cellular response to granulocyte macrophage colony-stimulating factor stimulus / activation of transmembrane receptor protein tyrosine kinase activity / Prolactin receptor signaling / cytokine binding / cell surface receptor signaling pathway via JAK-STAT / peptide hormone binding / mammary gland alveolus development / regulation of cell adhesion / Growth hormone receptor signaling / positive regulation of B cell proliferation / positive regulation of protein autophosphorylation / lactation / embryo implantation / endosome lumen / response to bacterium / cytokine-mediated signaling pathway / positive regulation of cold-induced thermogenesis / receptor complex / external side of plasma membrane / lipid binding / positive regulation of cell population proliferation / negative regulation of apoptotic process / protein kinase binding / cell surface / extracellular region / metal ion binding / plasma membrane 類似検索 - 分子機能
Long hematopoietin receptor, single chain, conserved site / Long hematopoietin receptor, single chain family signature. / Growth hormone/erythropoietin receptor, ligand binding / Erythropoietin receptor, ligand binding / : / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin-like fold 類似検索 - ドメイン・相同性
0.8 mM [U-13C; U-15N] PRLRTMD, 2 mM DSS, 0.05 % sodium azide, 560 mM DHPC, 20 mM sodium phosphate, 4 mM TCEP, 50 mM sodium chloride, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-13C; U-15N] PRLRTMD, 2 mM DSS, 0.05 % sodium azide, 700 mM [U-2H] DHPC, 20 mM sodium phosphate, 4 mM TCEP, 50 mM sodium chloride, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.7 mM [U-15N] PRLRTMD, 2 mM DSS, 0.05 % sodium azide, 490 mM DHPC, 20 mM sodium phosphate, 4 mM TCEP, 50 mM sodium chloride, 90% H2O/10% D2O
90% H2O/10% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.8mM
PRLRTMD-1
[U-13C; U-15N]
1
2mM
DSS-2
1
0.05 %
sodium azide-3
1
560mM
DHPC-4
1
20mM
sodium phosphate-5
1
4mM
TCEP-6
1
50mM
sodium chloride-7
1
1mM
PRLRTMD-8
[U-13C; U-15N]
2
2mM
DSS-9
2
0.05 %
sodium azide-10
2
700mM
DHPC-11
[U-2H]
2
20mM
sodium phosphate-12
2
4mM
TCEP-13
2
50mM
sodium chloride-14
2
0.7mM
PRLRTMD-15
[U-15N]
3
2mM
DSS-16
3
0.05 %
sodium azide-17
3
490mM
DHPC-18
3
20mM
sodium phosphate-19
3
4mM
TCEP-20
3
50mM
sodium chloride-21
3
試料状態
イオン強度: 50 / pH: 7.2 / 圧: ambient / 温度: 310 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
800
1
Varian INOVA
Varian
INOVA
750
2
Bruker Avance
Bruker
AVANCE
900
3
-
解析
NMR software
名称
バージョン
開発者
分類
ARIA
2.3.2
Linge, O'DonoghueandNilges
構造決定
ARIA
2.3.2
Linge, O'DonoghueandNilges
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 10