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Open data
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Basic information
Entry | Database: PDB / ID: 2n2s | ||||||
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Title | NMR solution structure of the pheromone Ep-1 from Euplotes petzi | ||||||
![]() | pheromone Ep-1 | ||||||
![]() | SIGNALING PROTEIN / WATER-BORNE PHEROMONE / ALPHA-HELICAL PROTEIN / DISULPHIDE-RICH PROTEIN | ||||||
Function / homology | Pheromone Ep-1![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
![]() | Pedrini, B. / Vallesi, A. / Alimenti, C. / Luporini, P. | ||||||
![]() | ![]() Title: NMR solution structure of the pheromone Ep-1 from Euplotes petzi Authors: Vallesi, A. / Alimenti, C. / Pedrini, B. / Luporini, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 171.3 KB | Display | ![]() |
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PDB format | ![]() | 122 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.3 KB | Display | ![]() |
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Full document | ![]() | 609.4 KB | Display | |
Data in XML | ![]() | 26.7 KB | Display | |
Data in CIF | ![]() | 29.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3180.615 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Harvested in 2005 at Adelie Cove, Terra Nova Bay (Ross Sea, Antarctica), S74 46'/E164 00' Source: (natural) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.25 mM Ep, 95% H2O/5% D2O / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 0.25 mM / Component: Ep-1-1 |
Sample conditions | Ionic strength: 0.06 / pH: 6.0 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||
NMR constraints | NOE constraints total: 372 / NOE intraresidue total count: 71 / NOE long range total count: 61 / NOE medium range total count: 130 / NOE sequential total count: 110 | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 20 |