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Yorodumi- PDB-2n07: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic C... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n07 | ||||||
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Title | Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic alpha-Conotoxin Vc1.1 | ||||||
Components | Alpha-conotoxin Vc1A | ||||||
Keywords | TOXIN / Disulfide-Deleted Mutant / cVc1.1 | ||||||
Function / homology | Function and homology information host cell postsynaptic membrane / acetylcholine receptor inhibitor activity / ion channel regulator activity / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Conus victoriae (invertebrata) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Yu, R. / Seymour, V. / Berecki, G. / Jia, X. / Akcan, M. / Adams, D. / Kaas, Q. / Craik, D. | ||||||
Citation | Journal: To be Published Title: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic alpha-Conotoxin Vc1.1. Authors: Yu, R. / Seymour, V. / Berecki, G. / Jia, X. / Akcan, M. / Adams, D. / Kaas, Q. / Craik, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n07.cif.gz | 101 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n07.ent.gz | 79.9 KB | Display | PDB format |
PDBx/mmJSON format | 2n07.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n0/2n07 ftp://data.pdbj.org/pub/pdb/validation_reports/n0/2n07 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2263.407 Da / Num. of mol.: 1 / Fragment: UNP residues 50-66 / Mutation: C2H, C8F / Source method: obtained synthetically / Source: (synth.) Conus victoriae (invertebrata) / References: UniProt: P69747 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.7 mM cVc 1.1 mutant, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.7 mM / Component: cVc 1.1 mutant-1 |
Sample conditions | Ionic strength: 0 / pH: 4.5 / Pressure: ambient / Temperature: 280 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |