- PDB-2mv6: Solution structure of the transmembrane domain and the juxta-memb... -
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基本情報
登録情報
データベース: PDB / ID: 2mv6
タイトル
Solution structure of the transmembrane domain and the juxta-membrane domain of the Erythropoietin Receptor in micelles
要素
Erythropoietin receptor
キーワード
MEMBRANE PROTEIN / Micelles / Transmembrane domain / Erythropoietin receptor
機能・相同性
機能・相同性情報
erythropoietin receptor activity / Signaling by Erythropoietin / Erythropoietin activates STAT5 / Erythropoietin activates Phospholipase C gamma (PLCG) / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / hemopoiesis / decidualization / Erythropoietin activates RAS / brain development / cytokine-mediated signaling pathway ...erythropoietin receptor activity / Signaling by Erythropoietin / Erythropoietin activates STAT5 / Erythropoietin activates Phospholipase C gamma (PLCG) / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / hemopoiesis / decidualization / Erythropoietin activates RAS / brain development / cytokine-mediated signaling pathway / heart development / nuclear speck / external side of plasma membrane / positive regulation of cell population proliferation / signal transduction / extracellular region / identical protein binding / plasma membrane 類似検索 - 分子機能
Erythropoietin receptor / Long hematopoietin receptor, single chain, conserved site / Long hematopoietin receptor, single chain family signature. / Growth hormone/erythropoietin receptor, ligand binding / Erythropoietin receptor, ligand binding / Fibronectin type III domain / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin-like fold 類似検索 - ドメイン・相同性
20 mM sodium phosphate, 10 v/v D2O, 250 mM DPC, 0.5 mM [U-99% 15N] protein, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.5-1 mM [U-100% 13C; U-100% 15N] protein, 10 % D2O, 20 mM sodium phosphate, 200-400 mM DPC, 1 mM DTT, 90% H2O/10% D2O
90% H2O/10% D2O
試料
濃度 (mg/ml)
単位
構成要素
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
20mM
sodium phosphate-1
1
10v/v
D2O-2
1
250mM
DPC-3
1
0.5mM
entity-4
[U-99% 15N]
1
mM
entity-5
[U-100% 13C; U-100% 15N]
0.5-1
2
10 %
D2O-6
2
20mM
sodium phosphate-7
2
mM
DPC-8
200-400
2
1mM
DTT-9
2
試料状態
イオン強度: 20 / pH: 6.5 / 圧: ambient / 温度: 313 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
700
1
Bruker Avance
Bruker
AVANCE
600
2
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解析
NMR software
名称
開発者
分類
CYANA
Guntert, MumenthalerandWuthrich
構造決定
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
構造決定
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
NMRView
Johnson, OneMoonScientific
peakpicking
TopSpin
BrukerBiospin
collection
X-PLOR NIH
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: simulated annealing was started with 3500 and cool down to 100 K with 15,000 steps. Structure was energy minimized with powell energy minimization.
NMR constraints
NOE constraints total: 260 / NOE intraresidue total count: 78 / NOE long range total count: 0 / NOE medium range total count: 42 / NOE sequential total count: 140 / Hydrogen bond constraints total count: 17 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 40 / Protein psi angle constraints total count: 40
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20