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- PDB-2muk: 1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17 -

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Basic information

Entry
Database: PDB / ID: 2muk
Title1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17
ComponentsAuxin-responsive protein IAA17
KeywordsTRANSCRIPTION / Aux/IAA
Function / homology
Function and homology information


positive regulation of leaf senescence / response to auxin / auxin-activated signaling pathway / DNA-binding transcription factor activity / identical protein binding / nucleus
Similarity search - Function
AUX/IAA protein / AUX/IAA domain / AUX/IAA family / PB1 domain profile. / PB1 domain
Similarity search - Domain/homology
Auxin-responsive protein IAA17
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodSOLUTION NMR / simulated annealing
Model detailsfewest violations, model1
AuthorsSuh, J. / Han, M.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2014
Title: Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17.
Authors: Han, M. / Park, Y. / Kim, I. / Kim, E.H. / Yu, T.K. / Rhee, S. / Suh, J.Y.
History
DepositionSep 11, 2014Deposition site: BMRB / Processing site: RCSB
Revision 1.0Dec 17, 2014Provider: repository / Type: Initial release
Revision 1.1Jan 14, 2015Group: Database references
Revision 1.2May 1, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_nmr_software / pdbx_nmr_spectrometer / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _pdbx_nmr_spectrometer.model / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
X: Auxin-responsive protein IAA17


Theoretical massNumber of molelcules
Total (without water)12,8021
Polymers12,8021
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100structures with the least restraint violations
RepresentativeModel #1fewest violations

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Components

#1: Protein Auxin-responsive protein IAA17 / Auxin response 3 / Indoleacetic acid-induced protein 17


Mass: 12801.700 Da / Num. of mol.: 1 / Fragment: UNP residues 105-217
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: IAA17, AXR3, At1g04250, F19P19.31 / Production host: Escherichia coli (E. coli) / References: UniProt: P93830

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-15N HSQC
1213D HN(CA)CB
1313D CBCA(CO)NH

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Sample preparation

DetailsContents: 1 mM [U-99% 13C; U-99% 15N] Aux/IAA17, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
SampleConc.: 1 mM / Component: Aux/IAA17-1 / Isotopic labeling: [U-99% 13C; U-99% 15N]
Sample conditionsIonic strength: 0.01 / pH: 7.4 / Pressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AvanceBrukerAVANCE9001
Bruker AvanceBrukerAVANCE6002

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Processing

NMR software
NameDeveloperClassification
X-PLOR NIHSchwieters, Kuszewski, Tjandra and Clorestructure solution
X-PLOR NIHrefinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR representativeSelection criteria: fewest violations
NMR ensembleConformer selection criteria: structures with the least restraint violations
Conformers calculated total number: 100 / Conformers submitted total number: 20

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