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- PDB-2mkn: Structural Characterization of Interactions between the Double-St... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2mkn | ||||||
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Title | Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and dsRNA | ||||||
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![]() | RNA BINDING PROTEIN/RNA / Zinc finger / dsRNA-binding / RNA BINDING PROTEIN-RNA complex | ||||||
Function / homology | ![]() miRNA binding / double-stranded RNA binding / nucleolus / enzyme binding / RNA binding / zinc ion binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Wright, P. / Dyson, J. / Burge, R. / Martinez-Yamout, M. | ||||||
![]() | ![]() Title: Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and Nucleic Acids. Authors: Burge, R.G. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 49.7 KB | Display | ![]() |
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PDB format | ![]() | 31 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.7 KB | Display | ![]() |
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Full document | ![]() | 429.2 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Data in CIF | ![]() | 6.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 7030.144 Da / Num. of mol.: 1 / Fragment: UNP residues 181-224 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: RNA chain | Mass: 6157.673 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#3: RNA chain | Mass: 6049.712 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#4: Chemical | ChemComp-ZN / |
Compound details | THE HADDOCK STARTING RNA STRUCTURE IS AN IDEALIZED A-FORM HELIX AND WAS GENERATED FROM THE MAKE-NA SERVER. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 200-300 mM [U-95% 13C; U-95% 15N] protein, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Units: mM / Component: entity_1-1 / Isotopic labeling: [U-95% 13C; U-95% 15N] / Conc. range: 200-300 |
Sample conditions | Ionic strength: 55 / pH: 7.4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: HADDOCK MODEL OF JAZ ZINC FINGER 3 COMPLEXED WITH DSRNA. | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformers calculated total number: 100 / Conformers submitted total number: 1 |