Mass: 2458.615 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: synthesized using phosphoramidite chemistry
#2: DNA chain
DNA_(5'-D(*CP*AP*GP*(N4S)P*CP*GP*AP*C)-3')
Mass: 2581.905 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: synthesized using phosphoramidite chemistry
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-1H NOESY
1
2
1
2D 1H-1H COSY
1
3
1
2D 1H-1H TOCSY
1
4
1
2D 1H-13C HSQC
1
5
1
2D 1H-31P HSQC
2
6
2
2D 1H-1H NOESY
-
Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.5 mM DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3'), 0.5 mM DNA (5'-D(*CP*AP*GP*CP*(N4S)P*GP*AP*C)-3'), 100 % [U-100% 2H] D2O, 50 mM sodium chloride, 10 mM sodium phosphate, 0.1 mM EDTA, 100% D2O
100% D2O
2
0.5 mM DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3'), 0.5 mM DNA (5'-D(*CP*AP*GP*CP*(N4S)P*GP*AP*C)-3'), 10 % [U-100% 2H] D2O, 90 % H2O, 50 mM sodium chloride, 10 mM sodium phosphate, 0.1 mM EDTA, 90% H2O/10% D2O
90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.5mM
DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3')-1
1
0.5mM
DNA (5'-D(*CP*AP*GP*CP*(N4S)P*GP*AP*C)-3')-2
1
100 %
D2O-3
[U-100% 2H]
1
50mM
sodium chloride-4
1
10mM
sodium phosphate-5
1
0.1mM
EDTA-6
1
0.5mM
DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3')-7
2
0.5mM
DNA (5'-D(*CP*AP*GP*CP*(N4S)P*GP*AP*C)-3')-8
2
10 %
D2O-9
[U-100% 2H]
2
90 %
H2O-10
2
50mM
sodium chloride-11
2
10mM
sodium phosphate-12
2
0.1mM
EDTA-13
2
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
60
7
ambient
298K
2
60
7
ambient
283K
-
NMR measurement
NMR spectrometer
Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz
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