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- PDB-2mcg: THREE-DIMENSIONAL STRUCTURE OF A LIGHT CHAIN DIMER CRYSTALLIZED I... -
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Basic information
Entry | Database: PDB / ID: 2mcg | ||||||||||||
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Title | THREE-DIMENSIONAL STRUCTURE OF A LIGHT CHAIN DIMER CRYSTALLIZED IN WATER. CONFORMATIONAL FLEXIBILITY OF A MOLECULE IN TWO CRYSTAL FORMS | ||||||||||||
![]() | IMMUNOGLOBULIN LAMBDA DIMER MCG (LIGHT CHAIN) | ||||||||||||
![]() | IMMUNOGLOBULIN | ||||||||||||
Function / homology | ![]() CD22 mediated BCR regulation / immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / FCERI mediated Ca+2 mobilization ...CD22 mediated BCR regulation / immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / antigen binding / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / adaptive immune response / Potential therapeutics for SARS / immune response / extracellular space / extracellular region / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() | ||||||||||||
![]() | Ely, K.R. / Herron, J.N. / Edmundson, A.B. | ||||||||||||
![]() | ![]() Title: Three-dimensional structure of a light chain dimer crystallized in water. Conformational flexibility of a molecule in two crystal forms. Authors: Ely, K.R. / Herron, J.N. / Harker, M. / Edmundson, A.B. #1: ![]() Title: The Binding of Opioid Peptides to the Mcg Light Chain Dimer. Flexible Keys and Adjustable Locks Authors: Edmundson, A.B. / Ely, K.R. / Herron, J.N. / Cheson, B.D. #2: ![]() Title: Three-Dimensional Structure of the Orthorhombic Form of the Mcg Bence-Jones Dimer Authors: Ely, K.R. / Herron, J.N. / Edmundson, A.B. #3: ![]() Title: Small-Angle Neutron Scattering Study of Bence-Jones Protein Mcg. Comparison of Structures in Solution and in Crystal Authors: Schiffer, M. / Stevens, F.J. / Westholm, F.A. / Kim, S.S. / Carlson, R.D. #4: ![]() Title: Marked Structural Differences of the Mcg Bence-Jones Dimer in Two Crystal Systems Authors: Abola, E.E. / Ely, K.R. / Edmundson, A.B. #5: ![]() Title: Crystal Properties as Indicators of Conformational Changes During Ligand Binding or Interconversion of Mcg Light Chain Isomers Authors: Ely, K.R. / Firca, J.R. / Williams, K.J. / Abola, E.E. / Fenton, J.M. / Schiffer, M. / Panagiotopoulos, N.C. / Edmundson, A.B. #6: ![]() Title: Interconversion of Conformational Isomers of Light Chains in the Mcg Immunoglobulins Authors: Firca, J.R. / Ely, K.R. / Kremser, P. / Westholm, F.A. / Dorrington, K.J. / Edmundson, A.B. #7: ![]() Title: The Antibody Combining Site Authors: Capra, J.D. / Edmundson, A.B. #8: ![]() Title: Implications of Conformational Isomerism and Rotational Allomerism to the Binding of Small Molecules by the Mcg Bence-Jones Dimer Authors: Edmundson, A.B. / Abola, E.E. / Ely, K.R. / Firca, J.R. / Panagiotopoulos, N.C. / Schiffer, M. / Westholm, F.A. #9: ![]() Title: Conformational Isomerism,Rotational Allomerism, and Divergent Evolution in Immunoglobulin Light Chains Authors: Edmundson, A.B. / Ely, K.R. / Abola, E.E. / Schiffer, M. / Panagiotopoulos, N. / Deutsch, H.F. #10: ![]() Title: Rotational Allomerism and Divergent Evolution of Domains in Immunoglobulin Light Chains Authors: Edmundson, A.B. / Ely, K.R. / Abola, E.E. / Schiffer, M. / Panagiotopoulos, N. #11: ![]() Title: Binding of 2,4-Dinitrophenyl Compounds and Other Small Molecules to a Crystalline Lambda-Type Bence-Jones Dimer Authors: Edmundson, A.B. / Ely, K.R. / Girling, R.L. / Abola, E.E. / Schiffer, M. / Westholm, F.A. / Fausch, M.D. / Deutsch, H.F. #12: ![]() Title: Structure and Binding Properties of a Lambda-Type Bence-Jones Dimer Authors: Edmundson, A.B. / Ely, K.R. / Girling, R.L. / Abola, E.E. / Schiffer, M. / Westholm, F.A. #13: ![]() Title: Primary Structure of the Mcg Lambda Chain Authors: Fett, J.W. / Deutsch, H.F. #14: ![]() Title: Preparation and Properties of a Bence-Jones Dimer with Mercury Inserted Into the Interchain Disulfide Bond Authors: Ely, K.R. / Girling, R.L. / Schiffer, M. / Cunningham, D.E. / Edmundson, A.B. #15: ![]() Title: Structure of a Lambda-Type Bence-Jones Protein at 3.5-Angstroms Resolution Authors: Schiffer, M. / Girling, R.L. / Ely, K.R. / Edmundson, A.B. #16: ![]() Title: Structural Features of Immunoglobulin Light Chains Authors: Edmundson, A.B. / Schiffer, M. / Ely, K.R. / Wood, M.K. #17: ![]() Title: Structure of a Lambda-Type Bence-Jones Protein at 6-Angstroms Resolution Authors: Edmundson, A.B. / Schiffer, M. / Ely, K.R. / Wood, M.K. #18: ![]() Title: Crystallographic Studies of an Igg Immunoglobulin and the Bence-Jones Protein from One Patient Authors: Edmundson, A.B. / Schiffer, M. / Wood, M.K. / Hardman, K.D. / Ely, K.R. / Ainsworth, C.F. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.1 KB | Display | ![]() |
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PDB format | ![]() | 75.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 388.8 KB | Display | ![]() |
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Full document | ![]() | 473.3 KB | Display | |
Data in XML | ![]() | 23.1 KB | Display | |
Data in CIF | ![]() | 33.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 22833.064 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.93 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7.4 / Method: microdialysis / Details: solting-in | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 7 Å / Num. obs: 12906 / Rmerge(I) obs: 5 |
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Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2→7 Å / σ(I): 5 /
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Refinement step | Cycle: LAST / Resolution: 2→7 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 7 Å / Num. reflection obs: 12906 / σ(I): 5 / Rfactor obs: 0.187 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 8.76 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.3 Å |