+Open data
-Basic information
Entry | Database: PDB / ID: 2m4i | ||||||
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Title | Solution structure of Bacillus subtilis MinC N-terminal domain | ||||||
Components | Septum site-determining protein MinC | ||||||
Keywords | CELL CYCLE / MinC / Bacillus subtilis / FtsZ inhibitor | ||||||
Function / homology | Function and homology information negative regulation of protein polymerization / regulation of cell septum assembly / division septum assembly / cell morphogenesis Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing, torsion angle dynamics, refinement | ||||||
Model details | minimized average structure, model1 | ||||||
Model type details | minimized average | ||||||
Authors | Castellen, P. / Sforca, M.L. / Zeri, A.C.M. / Gueiros-Filho, F.J. | ||||||
Citation | Journal: To be Published Title: Solution structure of Bacillus subtilis MinC N-terminal domain Authors: Castellen, P. / Sforca, M.L. / Zeri, A.C.M. / Gueiros-Filho, F.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m4i.cif.gz | 657.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m4i.ent.gz | 550.2 KB | Display | PDB format |
PDBx/mmJSON format | 2m4i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m4i_validation.pdf.gz | 473.9 KB | Display | wwPDB validaton report |
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Full document | 2m4i_full_validation.pdf.gz | 998.8 KB | Display | |
Data in XML | 2m4i_validation.xml.gz | 135.5 KB | Display | |
Data in CIF | 2m4i_validation.cif.gz | 102.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m4/2m4i ftp://data.pdbj.org/pub/pdb/validation_reports/m4/2m4i | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12028.641 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 1-102 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Strain: 168 / Gene: BSU28000, minC / Production host: Escherichia coli (E. coli) / References: UniProt: Q01463 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 7.4 / Pressure: ambient atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing, torsion angle dynamics, refinement Software ordinal: 1 | |||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1257 / NOE intraresidue total count: 399 / NOE long range total count: 207 / NOE medium range total count: 235 / NOE sequential total count: 416 | |||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | |||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Maximum lower distance constraint violation: 0 Å / Maximum upper distance constraint violation: 0 Å / Representative conformer: 1 | |||||||||||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.58 Å / Distance rms dev error: 0.13 Å |