MCM complex / DNA duplex unwinding / helicase activity / single-stranded DNA binding / DNA replication / DNA helicase / ATP hydrolysis activity / ATP binding / identical protein binding 類似検索 - 分子機能
700 uM [U-100% 13C; U-100% 15N] C-terminus of the minichromosome maintenance protein MCM-1, 10 mM potassium phosphate-2, 150 mM sodium chloride-3, 0.05 w/v sodium azide-4, 90% H2O/10% D2O
90% H2O/10% D2O
2
700 uM [U-100% 13C; U-100% 15N] C-terminus of the minichromosome maintenance protein MCM-5, 10 mM potassium phosphate-6, 150 mM sodium chloride-7, 0.05 w/v sodium azide-8, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
700uM
C-terminus of the minichromosome maintenance protein MCM-1
[U-100% 13C; U-100% 15N]
1
10mM
potassium phosphate-2
1
150mM
sodium chloride-3
1
0.05w/v
sodium azide-4
1
700uM
C-terminus of the minichromosome maintenance protein MCM-5
[U-100% 13C; U-100% 15N]
2
10mM
potassium phosphate-6
2
150mM
sodium chloride-7
2
0.05w/v
sodium azide-8
2
試料状態
イオン強度: 160 / pH: 6.2 / 圧: ambient / 温度: 303 K
-
NMR測定
NMRスペクトロメーター
タイプ: Bruker AvanceIII / 製造業者: Bruker / モデル: AVANCE III / 磁場強度: 600 MHz