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- PDB-2m12: Solution structure of the ID3 stem loop of domain 1 of the ai5gam... -
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Basic information
Entry | Database: PDB / ID: 2m12 | ||||||||||||||||||||
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Title | Solution structure of the ID3 stem loop of domain 1 of the ai5gamma group II intron | ||||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / EBS1 / stem loop / ID3 | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / torsion angle dynamics | Model details | fewest violations, model3 | ![]() Popovic, M. / Greenbaum, N.L. | ![]() ![]() Title: Role of helical constraints of the EBS1-IBS1 duplex of a group II intron on demarcation of the 5' splice site. Authors: Popovic, M. / Greenbaum, N.L. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 145.8 KB | Display | ![]() |
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PDB format | ![]() | 123.7 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 397.6 KB | Display | ![]() |
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Full document | ![]() | 476.8 KB | Display | |
Data in XML | ![]() | 12.1 KB | Display | |
Data in CIF | ![]() | 15.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 7443.480 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: RNA stem loop | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR constraints | NOE constraints total: 287 / NOE intraresidue total count: 130 / NOE long range total count: 26 / NOE sequential total count: 99 | ||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |