[English] 日本語
![](img/lk-miru.gif)
- PDB-2lxs: Allosteric communication in the KIX domain proceeds through dynam... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2lxs | ||||||
---|---|---|---|---|---|---|---|
Title | Allosteric communication in the KIX domain proceeds through dynamic re-packing of the hydrophobic core | ||||||
![]() |
| ||||||
![]() | TRANSFERASE / CREB binding protein / mixed-lineage leukemia activation domain / binary complex | ||||||
Function / homology | ![]() protein-cysteine methyltransferase activity / response to potassium ion / [histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / unmethylated CpG binding / histone H3K4 trimethyltransferase activity / peptide lactyltransferase (CoA-dependent) activity / negative regulation of DNA methylation-dependent heterochromatin formation / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity ...protein-cysteine methyltransferase activity / response to potassium ion / [histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / unmethylated CpG binding / histone H3K4 trimethyltransferase activity / peptide lactyltransferase (CoA-dependent) activity / negative regulation of DNA methylation-dependent heterochromatin formation / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / regulation of short-term neuronal synaptic plasticity / regulation of smoothened signaling pathway / histone H3K18 acetyltransferase activity / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / N-terminal peptidyl-lysine acetylation / T-helper 2 cell differentiation / histone H3K27 acetyltransferase activity / NFE2L2 regulates pentose phosphate pathway genes / definitive hemopoiesis / NFE2L2 regulating MDR associated enzymes / MRF binding / histone H3K4 methyltransferase activity / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / Regulation of FOXO transcriptional activity by acetylation / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of gene expression by Hypoxia-inducible Factor / Nuclear events mediated by NFE2L2 / embryonic hemopoiesis / Regulation of NFE2L2 gene expression / negative regulation of transcription by RNA polymerase I / exploration behavior / NOTCH3 Intracellular Domain Regulates Transcription / anterior/posterior pattern specification / NFE2L2 regulating anti-oxidant/detoxification enzymes / TRAF6 mediated IRF7 activation / NFE2L2 regulating tumorigenic genes / histone methyltransferase complex / peptide-lysine-N-acetyltransferase activity / FOXO-mediated transcription of cell death genes / embryonic digit morphogenesis / homeostatic process / protein acetylation / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / positive regulation of transforming growth factor beta receptor signaling pathway / Formation of paraxial mesoderm / non-canonical NF-kappaB signal transduction / minor groove of adenine-thymine-rich DNA binding / Zygotic genome activation (ZGA) / stimulatory C-type lectin receptor signaling pathway / membrane depolarization / cellular response to nutrient levels / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / acetyltransferase activity / MLL1 complex / positive regulation of double-strand break repair via homologous recombination / histone acetyltransferase complex / Attenuation phase / regulation of cellular response to heat / negative regulation of fibroblast proliferation / histone acetyltransferase activity / homeostasis of number of cells within a tissue / histone acetyltransferase / spleen development / transcription initiation-coupled chromatin remodeling / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / cellular response to transforming growth factor beta stimulus / RORA activates gene expression / NPAS4 regulates expression of target genes / Regulation of lipid metabolism by PPARalpha / CD209 (DC-SIGN) signaling / Transferases; Transferring one-carbon groups; Methyltransferases / BMAL1:CLOCK,NPAS2 activates circadian gene expression / SUMOylation of transcription cofactors / post-embryonic development / Activation of gene expression by SREBF (SREBP) / Formation of the beta-catenin:TCF transactivating complex / circadian regulation of gene expression / Heme signaling / protein destabilization / lysine-acetylated histone binding / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / visual learning / protein modification process / Transcriptional activation of mitochondrial biogenesis / PPARA activates gene expression / Evasion by RSV of host interferon responses / Cytoprotection by HMOX1 / NOTCH1 Intracellular Domain Regulates Transcription / chromatin DNA binding / PKMTs methylate histone lysines / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / transcription coactivator binding / Transcriptional regulation of white adipocyte differentiation Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
![]() | Bruschweiler, S. / Schanda, P. / Konrat, R. / Tollinger, M. | ||||||
![]() | ![]() Title: Allosteric communication in the KIX domain proceeds through dynamic repacking of the hydrophobic core. Authors: Bruschweiler, S. / Konrat, R. / Tollinger, M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 674.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 567.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 548.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 65.9 KB | Display | |
Data in CIF | ![]() | 94 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 10353.856 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 587-673 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Protein/peptide | Mass: 2061.336 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 2840-2858 / Mutation: C841A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q03164, histone-lysine N-methyltransferase |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-
Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] entity_1-1, 2 mM [U-100% 13C; U-100% 15N] entity_2-2, 25 mM sodium chloride-3, 50 mM potassium phosphate-4, 1 mM sodium azide-5, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||||||||
Sample conditions | pH: 5.8 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer |
|
---|
-
Processing
NMR software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR constraints | NOE constraints total: 1016 / NOE intraresidue total count: 525 / NOE long range total count: 90 / NOE medium range total count: 127 / NOE sequential total count: 274 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |