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- PDB-2lwc: Met-enkephalin in DPMC SUV -

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Basic information

Entry
Database: PDB / ID: 2lwc
TitleMet-enkephalin in DPMC SUV
ComponentsMet-enkephalin
KeywordsNEUROPEPTIDE / SUV DMPC
Function / homology
Function and homology information


synaptic vesicle lumen / chromaffin granule lumen / opioid peptide activity / aggressive behavior / positive regulation of behavioral fear response / general adaptation syndrome, behavioral process / symmetric synapse / neuronal dense core vesicle lumen / synaptic signaling via neuropeptide / response to epinephrine ...synaptic vesicle lumen / chromaffin granule lumen / opioid peptide activity / aggressive behavior / positive regulation of behavioral fear response / general adaptation syndrome, behavioral process / symmetric synapse / neuronal dense core vesicle lumen / synaptic signaling via neuropeptide / response to epinephrine / cell body fiber / G protein-coupled opioid receptor signaling pathway / cellular response to vitamin D / sensory perception / neuropeptide hormone activity / startle response / transmission of nerve impulse / response to immobilization stress / locomotory exploration behavior / neuropeptide signaling pathway / cellular response to transforming growth factor beta stimulus / behavioral fear response / glial cell proliferation / axon terminus / sensory perception of pain / Peptide ligand-binding receptors / cellular response to cAMP / response to nicotine / Post-translational protein phosphorylation / response to calcium ion / cellular response to virus / response to toxic substance / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / osteoblast differentiation / response to estradiol / cellular response to oxidative stress / response to ethanol / response to lipopolysaccharide / G alpha (i) signalling events / perikaryon / chemical synaptic transmission / response to hypoxia / endoplasmic reticulum lumen / neuronal cell body / dendrite / signal transduction / extracellular region / plasma membrane
Similarity search - Function
Proenkephalin A / Opioid neuropeptide precursor / Vertebrate endogenous opioids neuropeptide / Endogenous opioids neuropeptides precursors signature.
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / DGSA-distance geometry simulated annealing
AuthorsMouret, L.
CitationJournal: To be Published
Title: Menk in DMPC SUV
Authors: Mouret, L.
History
DepositionJul 27, 2012Deposition site: BMRB / Processing site: RCSB
Revision 1.0Aug 7, 2013Provider: repository / Type: Initial release
Revision 1.1Jun 14, 2023Group: Data collection / Database references / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_nmr_software / pdbx_nmr_spectrometer
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_nmr_data / _pdbx_nmr_software.name / _pdbx_nmr_spectrometer.model
Revision 1.2May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 / Item: _database_2.pdbx_DOI

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Met-enkephalin


Theoretical massNumber of molelcules
Total (without water)5741
Polymers5741
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100target function
RepresentativeModel #1closest to the average

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Components

#1: Protein/peptide Met-enkephalin


Mass: 573.662 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P01210

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H NOESY
1212D 1H-1H COSY
1312D 1H-1H TOCSY

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Sample preparation

DetailsContents: 0.5 mM menk, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O
SampleConc.: 0.5 mM / Component: menk-1
Sample conditionsIonic strength: 0 / pH: 3 / Pressure: ambient / Temperature: 303 K

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NMR measurement

NMR spectrometerType: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 500 MHz

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Processing

NMR softwareName: CNS / Developer: Brunger, Adams, Clore, Gros, Nilges and Read / Classification: refinement
RefinementMethod: DGSA-distance geometry simulated annealing / Software ordinal: 1
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20

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