regulation of defense response to fungus / negative regulation of defense response to bacterium / response to ethylene / response to salicylic acid / response to jasmonic acid / defense response / sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / DNA binding ...regulation of defense response to fungus / negative regulation of defense response to bacterium / response to ethylene / response to salicylic acid / response to jasmonic acid / defense response / sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / DNA binding / nucleus / metal ion binding Similarity search - Function
Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
-
Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-1H NOESY
1
2
1
2D 1H-1H TOCSY
1
3
1
2D DQF-COSY
1
4
2
2D 1H-15N HSQC
1
5
2
3D HNCA
1
6
2
3DHN(CO)CA
1
7
2
3D HN(CA)CB
1
8
2
3DCBCA(CO)NH
1
9
2
3D 1H-15N NOESY
1
10
2
3D 1H-15N TOCSY
2
11
3
2D 1H-15N HSQC
3
12
4
2D 1H-15N HSQC
3
13
4
2D 1H-15N IPAP-HSQC
3
14
5
2D 1H-15N HSQC
3
15
5
2D 1H-15N IPAP-HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.4-1.0 mM WRKY domain-1, 0.4-1.0 mM W-box DNA chain 1-2, 0.4-1.0 mM W-box DNA chain 2-3, 20 mM potassium phosphate-4, 200 mM potassium chloride-5, 20 uM zinc chloride-6, 1 mM [U-2H] DTT-7, 50 uM DSS-8, 95% H2O/5% D2O
95% H2O/5% D2O
2
0.4-1.0 mM [U-13C; U-15N] WRKY domain-9, 0.4-1.0 mM W-box DNA chain 1-10, 0.4-1.0 mM W-box DNA chain 2-11, 20 mM potassium phosphate-12, 200 mM potassium chloride-13, 20 uM zinc chloride-14, 1 mM [U-2H] DTT-15, 50 uM DSS-16, 95% H2O/5% D2O
95% H2O/5% D2O
3
0.4-1.0 mM [U-13C; U-15N] WRKY domain-17, 0.4-1.0 mM W-box DNA chain 1-18, 0.4-1.0 mM W-box DNA chain 2-19, 20 mM potassium phosphate-20, 200 mM potassium chloride-21, 20 uM zinc chloride-22, 1 mM [U-2H] DTT-23, 50 uM DSS-24, 100% D2O
100% D2O
4
0.4-1.0 mM [U-15N] WRKY domain-25, 0.4-1.0 mM [U-15N]-T W-box DNA chain 1-26, 0.4-1.0 mM [U-15N]-T W-box DNA chain 2-27, 20 mM potassium phosphate-28, 200 mM potassium chloride-29, 20 uM zinc chloride-30, 1 mM [U-2H] DTT-31, 50 uM DSS-32, 95% H2O/5% D2O
95% H2O/5% D2O
5
0.4-1.0 mM [U-15N] WRKY domain-33, 0.4-1.0 mM [U-15N]-T W-box DNA chain 1-34, 0.4-1.0 mM [U-15N]-T W-box DNA chain 2-35, 20 mM potassium phosphate-36, 200 mM potassium chloride-37, 20 uM zinc chloride-38, 1 mM [U-2H] DTT-39, 50 uM DSS-40, 12 mg/mL Pf1 phage-41, 95% H2O/5% D2O
95% H2O/5% D2O
Sample
Conc. (mg/ml)
Units
Component
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
WRKY domain-1
0.4-1.0
1
mM
W-box DNA chain 1-2
0.4-1.0
1
mM
W-box DNA chain 2-3
0.4-1.0
1
20mM
potassium phosphate-4
1
200mM
potassium chloride-5
1
20uM
zinc chloride-6
1
1mM
DTT-7
[U-2H]
1
50uM
DSS-8
1
mM
WRKY domain-9
[U-13C; U-15N]
0.4-1.0
2
mM
W-box DNA chain 1-10
0.4-1.0
2
mM
W-box DNA chain 2-11
0.4-1.0
2
20mM
potassium phosphate-12
2
200mM
potassium chloride-13
2
20uM
zinc chloride-14
2
1mM
DTT-15
[U-2H]
2
50uM
DSS-16
2
mM
WRKY domain-17
[U-13C; U-15N]
0.4-1.0
3
mM
W-box DNA chain 1-18
0.4-1.0
3
mM
W-box DNA chain 2-19
0.4-1.0
3
20mM
potassium phosphate-20
3
200mM
potassium chloride-21
3
20uM
zinc chloride-22
3
1mM
DTT-23
[U-2H]
3
50uM
DSS-24
3
mM
WRKY domain-25
[U-15N]
0.4-1.0
4
mM
W-box DNA chain 1-26
[U-15N]-T
0.4-1.0
4
mM
W-box DNA chain 2-27
[U-15N]-T
0.4-1.0
4
20mM
potassium phosphate-28
4
200mM
potassium chloride-29
4
20uM
zinc chloride-30
4
1mM
DTT-31
[U-2H]
4
50uM
DSS-32
4
mM
WRKY domain-33
[U-15N]
0.4-1.0
5
mM
W-box DNA chain 1-34
[U-15N]-T
0.4-1.0
5
mM
W-box DNA chain 2-35
[U-15N]-T
0.4-1.0
5
20mM
potassium phosphate-36
5
200mM
potassium chloride-37
5
20uM
zinc chloride-38
5
1mM
DTT-39
[U-2H]
5
50uM
DSS-40
5
12mg/mL
Pf1 phage-41
5
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
200
6
ambient
303K
2
200
6
ambient
283K
3
200
6
ambient
298K
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
750
1
Bruker DMX
Bruker
DMX
500
2
-
Processing
NMR software
Name
Version
Developer
Classification
CNS
1.1
Brunger, Adams, Clore, Gros, NilgesandRead
structuresolution
CNS
1.1
Brunger, Adams, Clore, Gros, NilgesandRead
refinement
Felix
2000
AccelrysSoftwareInc.
dataanalysis
TALOS+
Shen, Delaglio, Cornilescu, Bax
dataanalysis
Refinement
Method: simulated annealing / Software ordinal: 1
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1
+
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