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- PDB-2lar: DNA / RNA Hybrid containing a central stereo specific Rp borano p... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2lar | ||||||
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Title | DNA / RNA Hybrid containing a central stereo specific Rp borano phosphate linkage | ||||||
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![]() | DNA/RNA / nucleic acid heteroduplex / RNase H1 activity / DNA-RNA complex | ||||||
Function / homology | DNA / RNA![]() | ||||||
Method | SOLUTION NMR / molecular dynamics, matrix relaxation | ||||||
Model details | structure with the lowest restraint violations, model 1 | ||||||
![]() | Johnson, C.N. / Spring, A.M. / Shaw, B.R. / Germann, M.W. | ||||||
![]() | ![]() Title: Structural basis of the RNase H1 activity on stereo regular borano phosphonate DNA/RNA hybrids. Authors: Johnson, C.N. / Spring, A.M. / Sergueev, D. / Shaw, B.R. / Germann, M.W. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 22.3 KB | Display | ![]() |
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PDB format | ![]() | 12.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 334 KB | Display | ![]() |
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Full document | ![]() | 339.1 KB | Display | |
Data in XML | ![]() | 2.7 KB | Display | |
Data in CIF | ![]() | 3.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 2743.637 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: RNA chain | Mass: 2854.782 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 50 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: molecular dynamics, matrix relaxation / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: structure with the lowest restraint violations | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structure with the lowest restraint violations Conformers calculated total number: 10 / Conformers submitted total number: 1 |