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- PDB-2l6c: Solution structure of desulfothioredoxin from Desulfovibrio vulga... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2l6c | ||||||
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Title | Solution structure of desulfothioredoxin from Desulfovibrio vulgaris Hildenborough in its oxidized form | ||||||
![]() | Thioredoxin | ||||||
![]() | OXIDOREDUCTASE / thioredoxin fold | ||||||
Function / homology | ![]() Glutaredoxin / Glutaredoxin domain profile. / Thioredoxin / Thioredoxin domain profile. / Thioredoxin domain / Glutaredoxin / Glutaredoxin / Thioredoxin-like superfamily / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Garcin, E.B. / Bornet, O. / Sebban-Kreuzer, C. / Guerlesquin, F. | ||||||
![]() | ![]() Title: Structural and mechanistic insights into unusual thiol disulfide oxidoreductase. Authors: Garcin, E.B. / Bornet, O. / Elantak, L. / Vita, N. / Pieulle, L. / Guerlesquin, F. / Sebban-Kreuzer, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 580.5 KB | Display | ![]() |
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PDB format | ![]() | 502 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 358.7 KB | Display | ![]() |
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Full document | ![]() | 449 KB | Display | |
Data in XML | ![]() | 27.5 KB | Display | |
Data in CIF | ![]() | 51.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 12384.338 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1 mM [U-99% 13C; U-99% 15N] oxidized desulfothioredoxin, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM / Component: oxidized desulfothioredoxin / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 0.1 / pH: 5.5 / Pressure: ambient / Temperature: 290 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |